Glucose Dehydrogenase (GDH)
Bayani
Glutamate Dehydrogenase (GDH) wani enzyme ne na mitochondrial wanda ke haifar da reversible oxidative deamination na glutamate zuwa a-ketoglutarate kuma yana aiki azaman hanyar haɗi tsakanin hanyoyin anabolic da catabolic.A cikin dabbobi masu shayarwa, GDH yana ƙarƙashin ƙa'idodin allosteric kuma yana da babban aiki a hanta, koda, kwakwalwa, da pancreas.Ayyukan GDH a cikin jini za a iya amfani da su don bambanta tsakanin cututtukan hanta saboda kumburin hanta, wanda ba ya nuna aikin GDH mai girma, da cututtuka da ke haifar da necrosis na hepatocyte, wanda ke haifar da hawan GDH.
Ayyukan GDH an ƙaddara ta hanyar nazarin enzyme guda biyu wanda glutamate ke cinyewa ta hanyar GDH yana haifar da NADH, wanda ke amsawa tare da binciken da ke samar da samfurin launi (450 nm) daidai da ayyukan GDH.Ɗaya daga cikin naúrar GDH shine adadin enzyme wanda zai samar da 1.0 mmle na NADH a minti daya a pH 7.6 a 37 ° C
Tsarin Sinadarai
Hanyar amsawa
D-Glucose + mai karɓa → D-Glucono-1,5-lactone + rage mai karɓa
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Bayani | Farin amorphous foda, lyophilized |
| Ayyuka | ≥160U/mg |
| Tsarki (SDS-SHAFIN) | ≥90% |
| Solubility (10 MG foda / ml) | Share |
| Gurɓataccen enzymes | |
| Glucose dehydrogenase (NAD) | ≤0.02% |
| Hexokinase | ≤0.02% |
| A-Glucosidase | ≤0.02% |
Sufuri da ajiya
Sufuri: Fakitin kankara
Ajiya:Adana a -25 ~ -15°C(Logon lokaci), 2-8°C (gajeren lokaci)
An shawarar sake gwadawaRayuwa: watanni 18
