M-MLV Reverse Transcriptase (Glycerol kyauta)
Rubutun juzu'i na lyophilizable.Ana iya amfani da shi zuwa fasahar lyophilization na ƙasa yayin da yake riƙe babban aikin juzu'i da kwanciyar hankali.Wannan samfurin ba ya ƙunshi abubuwan haɓakawa, da fatan za a ƙara naku idan an buƙata.
Abubuwan da aka gyara
| Bangaren | HC2005 A-01 (10,000U) | HC2005 A-02 (40,000U) |
| Reverse Transcriptase (Glycerol Kyauta) (200U/μL) | 50 μl | 200 μl |
| 5 × Maɓalli | 200 μl | 800 ml |
Aikace-aikace:
Yana dacewa don halayen RT-qPCR mataki ɗaya.
Yanayin Ajiya
Adana a -30 ~ -15°C da kuma jigilar kaya a ≤0°C.
Ma'anar Naúrar
An bayyana raka'a ɗaya (U) azaman adadin enzyme wanda ya haɗa 1 nmol na dTTP cikin kayan acid-insoluble a cikin 10mins a 37°C, tare da Poly(rA) · Oligo (dT) azaman samfuri/primer.
Bayanan kula
Don bincike kawai amfani.Ba don amfani a cikin hanyoyin bincike ba.
1.Da fatan za a tsaftace yankin gwaji;Sanya safar hannu da abin rufe fuska;Yi amfani da abubuwan amfani marasa RNase kamar bututun centrifuge da tukwici na pipette.
2.Ci gaba da RNA akan kankara don guje wa lalacewa.
3.Ana ba da shawarar samfuran RNA masu inganci don cimma ingantaccen juzu'i mai ƙarfi.
