Hotstart Taq DNA Polymerase (5u/ul)
Taq DNA Polymerase ne mai zafi fara DNA polymerase tare da biyu tarewa ta biyu antibodies.Wannan samfurin ba kawai toshe 5′→3′ polymerase ayyuka na Taq DNA polymerase, amma kuma toshe 5′→3′exonuclease aiki.Dumama na daƙiƙa 30 a zafin jiki na pre-denaturation na iya kashe gaba ɗaya antibody kuma ya saki ayyukan DNA polymerase da ayyukan exonuclease.Halayen toshewar sau biyu ba wai kawai zai iya hana haɓakar ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙaƙƙarfan haɓakawa da ke haifar da rashin daidaituwa ko madaidaicin dimer ba, amma kuma yadda ya kamata ya hana raguwar siginar walƙiya da lalacewa ta hanyar lalata bincike, ta yadda za a sanya in vitro gano reagent ya fi karko yayin sufuri ko amfani da shi a daki. zafin jiki.
Abubuwan da aka gyara
| Bangaren | HC1012B (250U) | HC1012B (1000U) | HC1012B (10000U) | HC1012B (25000U) |
| Taq DNA Polymerase(5 U/μL) | 50 μl | 200 μl | 2 ml | ml 5 |
Yanayin Ajiya
Ana jigilar samfurin tare da busassun ƙanƙara kuma ana iya adana shi a -25 ° C ~ -15 ° C na shekaru 2.
Ƙayyadaddun bayanai
| Polymerase | Taq DNA Polymerase |
| Tsafta | ≥ 95% (SDS-PAGE) |
| Zafafan Farawa | Wurin Farawa Mai Kyau |
| Saurin amsawa | Daidaitawa |
| Ayyukan Exonuclease | 5'→3' |
Umarni
Saitin Amsa
| Abubuwan da aka gyara | girma (μL) | Ƙarshe Tattaunawa |
| 2× Buffera | 25 | 1× |
| Haɗin farko/Bincikeb | × | 0.1 μmol/L-0.5 μmol/L |
| Hotstart Taq Polymerase (5U/μL) | 1.2 | 0.12 U/μL |
| Samfurin DNAc | × | 0.1-100 ng |
| ddH2O | Har zuwa 50 | - |
Bayanan kula:
1) Dangane da ƙayyadaddun aikace-aikacen gwaji, ana buƙata don shirya buffer mai dacewa daidai.
2) Adadin DNA da ƙaddamar da bincike ko abubuwan da aka tsara ana ba da shawarar ƙira.Za'a iya daidaita mafi kyawun maida hankali bisa ga takamaiman yanayin gwaji.
Ƙa'idar hawan keke na thermal
| Mataki | Zazzabi(°C) | Lokaci | Zagaye |
| Pre-denaturation | 95 ℃ | 5 min | 1 |
| Denaturation | 95 ℃ | dakika 15 | 45 |
| Annealing / Extension | 60 ℃a | 30 secondsb |
Bayanan kula:
1) Ana daidaita yawan zafin jiki bisa ga ƙimar Tm na abubuwan da aka tsara.
2) Na'urorin qPCR daban-daban suna buƙatar lokacin siginar siginar haske daban-daban, da fatan za a saita bisa ga mafi ƙarancin lokacin.
Bayanan kula
Da fatan za a saka PPE da ake buƙata, irin wannan rigar lab da safar hannu, don tabbatar da lafiyar ku da amincin ku!
