Kit ɗin bincike na Mataki ɗaya na RT-qPCR
Bayani
U+ Ɗayan Mataki na RT-qPCR Probe Kit (Glycerol-kyauta) mai ba da glycerol mara izini mataki ɗaya na RT-qPCR reagent ta amfani da RNA azaman samfuri (kamar cutar RNA), wanda ya dace da haɓakawa da ƙira na samfuran lyophilized.Wannan samfurin yana haɗe mafi kyawun aiki na sadaukarwar mataki ɗaya na Reverse Transcriptase da farkon farawa Champagne Taq DNA Polymerase, tare da ingantaccen daskare-bushewar sinadarai, wanda ke da ingantaccen haɓakawa, daidaito da ƙayyadaddun, kuma ya dace da bushewa daban-daban. matakai. Bugu da ƙari, an gabatar da tsarin rigakafin dUTP / UDG a cikin reagent, wanda zai iya aiki a dakin da zafin jiki, kawar da tasiri na haɓaka samfurin haɓakawa akan qPCR, kuma tabbatar da daidaiton sakamakon.
Ka'idojin asali na RT-qPCR
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai | Sakamako |
| (SDS PAGE) Tsaftar Hannun Enzyme (SDS PAGE) | ≥95% | Wuce |
| Ayyukan Endonuclease | Ba a gano ba | Wuce |
| Ayyukan Exodulease | Ba a gano ba | Wuce |
| Ayyukan Rnase | Ba a gano ba | Wuce |
| Ragowar E.coli DNA | 1 kwafi/60 | Wuce |
| Tsare-tsare-Tsarin Aikin Assay | 90%≤110% | Wuce |
Abubuwan da aka gyara
| Abubuwan da aka gyara | 100rxn | 1,000 rns | 5,000 rxn |
| ddH2O mara kyauta | 2*1 ml | ml 20 | 100 ml |
| 5* mixing mataki daya | 600 μl | 6*1 ml | ml 30 |
| Haɗin enzyme mataki ɗaya | 150 μl | 2*750ml | 7.5ml ku |
| 50* ROX mai magana Dye 1 | 60ml ku | 600 μl | 3*1 ml |
| 50* ROX mai magana Dye 2 | 60ml ku | 600 μl | 3*1 ml |
a.Buffer Mataki ɗaya ya haɗa da dNTP Mix da Mg2+.
b.Enzyme Mix yana ƙunshe da baya
transcriptase, Hot Start Taq DNA polymerase (gyaran antibody) da RNase inhibitor.
c.Ana amfani dashi don gyara kuskuren sinal fluorescene tsakanin rijiyoyi daban-daban.
c.ROX: Kuna buƙatar zaɓar daidaitawa bisa ga ƙirar kayan aikin gwaji.
Aikace-aikace
Gano QPCR
Shipping da Adana
Sufuri:Fakitin kankara
Yanayin Ajiya:Adana a -20 ℃.
Rayuwar Shief:watanni 18
