2 × PCR Super Mix (tare da Dye)

2× PCR Master Mix ya ƙunshi Taq DNA Polymerase, dNTPs, da sauran abubuwan da ake buƙata na PCR.The Master Mix yana da kwanciyar hankali na tsawon watanni 3 a 4 ℃ tare da na'urorin daidaitawar mu.Maganin haɗakarwa an inganta shi don PCR na al'ada kuma yana shirye don amfani ta ƙara samfuri na DNA da maɓalli.Ana iya loda samfuran PCR kai tsaye don electrophoresis tare da rini mai shuɗi na bromophenol da aka riga aka ɗora.Abubuwan da aka haɓaka sun ƙunshi fitowar 3'-dA kuma ana iya haɗa su cikin sauƙi cikin T vector.2 × PCR Master Mix yana sauƙaƙe tsarin PCR kuma yana rage gurɓatawa.

Yanayin Ajiya

Ya kamata a adana samfuran a -25 ℃ ~ -15 ℃ na shekaru 2.

Ƙayyadaddun bayanai

Umarni

1.Tsarin martani

2.Ƙarfafa Ƙarfafawa

Lura:

1) Yin amfani da samfur: 50-200ng DNA na genomic;0.1-10ng DNA plasmid.

2) Mg²⁺maida hankali: Wannan samfurin ya ƙunshi 3mM na MgCl2, dace da yawancin halayen PCR.

3) Zazzaɓin zafi: Da fatan za a koma zuwa ƙimar Tm na ka'idar.Za'a iya saita zafin jiki na annealing zuwa 2-5 ℃ ƙasa da ƙimar ƙa'idar farko.

4) Lokacin tsawaitawa: Don gano ƙwayoyin ƙwayoyin cuta, ana ba da shawarar 30 sec/kb.Don cloning gene, ana bada shawarar 60 sec/kb.

Bayanan kula

1.Abubuwan PCR tare da 2 × PCR Master Mix ba su dace da polyacrylamide gel electrophoresis ba.

2.Don amincin ku da lafiyar ku, da fatan za a sa riguna na lab da safofin hannu masu zubarwa don aiki.

3.Ana amfani dashi don amfani da bincike KAWAI!