RT-LAMP Fluorescent (ƙwallon da aka yi wa ado)
RT-LAMP Fluorescent Master Mix (Lyophilized Foda) yana ƙunshe da buffer amsawa, Bst DNA polymerase da juzu'i mai jurewa juzu'i, kariyar lyophilized, da rini mai kyalli.Samfurin yana cikin nau'i na beads na lyophilized, kuma kawai firamare da samfura suna buƙatar ƙara don amfani.
Bangaren Reagent
RT-LAMP Fluorescent Master Mix (Foda Lyophilized)
Amfani
Don DNA ko RNA isothermal amplification
Yanayin Ajiya
Transport da adana a 2 ~ 8 ° C.
Rayuwar rayuwa: watanni 12.
Yarjejeniya
1. Fitar da daidai adadin beads lyophilized bisa ga adadin gwaje-gwaje.
2. Shirye-shiryen tsarin amsawa
| Bangaren | Sashi |
| RT-LAMP Fluorescent Master Mix (Fowdar Lyophilized) | 2 gwanda |
| Rini mai haske | x μl |
| 10 × Farko Mix a | 5 μl |
| Samfuran DNA/RNA b | n μl |
| Jimlar | 50 μl |
Bayanan kula:
1) a.10 × Primer Mix : 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Madauki F / B;
2) b.Ana ba da shawarar samfurin nucleic acid a narkar da shi a cikin ruwan DEPC.
Abubuwan da aka bayar na Hyasen Biotechnology Co., Ltd
3) Shirin mayar da martani
| Zazzabi | Lokaci | Zagaye |
| 65 ℃ | 60s a | 30 |
Bayanan kula:
1. Tattara alamar kyalli
Bayanan kula
1. Za a iya inganta yawan zafin jiki tsakanin 62 ° C da 68 ° C bisa ga firam.yanayi;
2. Gwajin ya kamata ya daidaita aikin, ciki har da shirye-shiryen tsarin amsawa,samfurin sarrafawa da dosing;
3. Don kauce wa gurɓatawa, ana bada shawara don shirya tsarin amsawa a cikin tebur mai tsabtakuma ƙara samfuri zuwa murfin hayaƙi a cikin wasu ɗakuna don guje wa tsangwama na ƙarya.
