2×Rapid Taq Super Mix
Cat No: HCR2016A
2 × Rapid Taq Super Mix ya dogara ne akan ingantaccen Taq DNA Polymerase, yana ƙara haɓakar haɓaka mai ƙarfi, haɓaka haɓaka haɓakawa da ingantaccen tsarin buffer, tare da ingantaccen ingantaccen haɓakawa.Gudun haɓakawa na hadaddun samfura irin su genome a cikin 3kb ya kai 1-3 sec/kb, kuma na samfura masu sauƙi kamar plasmids a cikin 5 kb ya kai 1 sec/kb.Wannan samfurin zai iya adana lokacin amsawar PCR sosai.A lokaci guda, haɗe-haɗe ya ƙunshi dNTP da Mg2+, waɗanda za a iya haɓaka su kawai ta hanyar ƙara abubuwan farko da samfuri, wanda kuma yana sauƙaƙa matakan aiki na gwaji.Bugu da ƙari, haɗe-haɗe yana ƙunshe da rini mai nuna alama na electrophoretic, wanda zai iya zama kai tsaye electrophoresis bayan amsawa.Wakilin kariyar a cikin wannan samfurin yana sa haɗin gwiwa ya ci gaba da aiki mai ƙarfi bayan daskare da narke mai maimaitawa.Ƙarshen 3'-ƙarshen A na samfurin PCR ana iya haɗa shi cikin sauƙi a cikin T vector.
Abubuwan da aka gyara
2×Rapid Taq Super Mix
Yanayin Ajiya
Ya kamata a adana samfuran PCR Master Mix a -25 ~ -15 ℃ na shekaru 2.
Ƙayyadaddun bayanai
| Ƙayyadaddun samfur | Rapid Taq Super Mix |
| Hankali | 2× |
| Zafafan Farawa | Wurin Farawa Mai Kyau |
| Tsayawa | 3'- A |
| Gudun amsawa | Mai sauri |
| Girman (samfurin ƙarshe) | Har zuwa 15 kb |
| Sharuɗɗan sufuri | Busasshen ƙanƙara |
Umarni
1. Tsarin amsawa (50 μL)
| Abubuwan da aka gyara | Girman (μL) |
| Samfurin DNA* | dace |
| Gabatarwa (10 μmol/L) | 2.5 |
| Mai juyawa (10 μmol/L) | 2.5 |
| 2×Rapid Taq Super Mix | 25 |
| ddH2O | zuwa 50 |
2.Ƙarfafa Ƙarfafawa
| Matakan zagayowar | Zazzabi (°C) | Lokaci | Zagaye |
| Predenaturation | 94 | 3 min | 1 |
| Denaturation | 94 | 10 dakika |
28-35 |
| Annealing | 60 | dakika 20 | |
| Tsawaita | 72 | 1-10 sec/kb |
An shawarar yin amfani da samfuri daban-daban:
| Nau'in samfuri | Yankin amfani da yanki (tsarin amsa 50 μL) |
| Genomic DNA ko E. coli ruwa | 10-1,000 ng |
| Plasmid ko kwayar cutar DNA | 0.5-50 ng |
| cDNA | 1-5 µL (ba fiye da 1/10 na jimlar yawan amsawar PCR ba) |
| An shawarar yin amfani da samfuri daban-daban | |
Bayanan kula:
1.Amfani da Reagent: cikakken narke da gauraya kafin amfani.
2. Annealing zafin jiki: The annealing zafin jiki ne na duniya Tm darajar, kuma za a iya saita 1-2℃ kasa da na farko Tm darajar.
3. Saurin haɓakawa: Saita 1 sec / kb don samfurori masu rikitarwa irin su genome da E. coli a cikin 1 kb;saita 3 sec/kb don hadaddun samfura kamar 1-3 kb genome da E. coli;saita 10 sec/kb don hadaddun samfura sama da 3kb genome da E. coli.Kuna iya saita ƙimar zuwa 1 sec/kb don samfuri mai sauƙi kamar plasmid ƙasa da 5 kb, 5 sec/kb don samfuri mai sauƙi kamar plasmid tsakanin 5 da 10 kb, da 10 sec/kb don samfuri mai sauƙi. kamar plasmid girma fiye da 10 kb.
Bayanan kula
1. Don amincin ku da lafiyar ku, da fatan za a sa riguna na lab da safar hannu da za a iya zubarwa don aiki.
2. Wannan samfurin don yin amfani da bincike ne kawai!
