Mai launi mai launi na RT-LAMP (Kwallon Lyophilized)
Wannan samfurin yana ƙunshe da buffer, RT-Enzymes Mix (Bst DNA polymerase and heat-resistant reverse transcriptase), masu kare lyophilized da abubuwan rini na chromogenic.Samfurin shine nau'in ƙwallon lyophilized, ana amfani da shi kawai tare da firamare da samfuri.Wannan kit ɗin yana ba da sauri, bayyananniyar gano gani na haɓakawa, wanda aka nuna mummunan amsa a cikin ja kuma ana nuna sakamako mai kyau ta canji zuwa rawaya.
Bangaren
RT-LAMP Mai launi mai launi Master Mix (Lyophilized beads)
Aikace-aikace
Don DNA ko RNA isothermal amplification.
Yanayin Ajiya
Ana jigilar kaya da adanawa a 2 ~ 8 ℃.Samfurin yana aiki na watanni 12.
Yarjejeniya
1.Cire madaidaicin lambar Lyophilized beads foda gwargwadon adadin gwaje-gwaje.
2.Shirya mix na amsawa
| Bangaren | Ƙarar |
| RT-LAMP Mai launi mai launi Master Mix (Lyophilized beads) | 1 guda (2 beads) |
| 10 × Farko Mixa | 5 μl |
| Samfuran DNA/RNA b | 45 ml |
Bayanan kula:
1. 10 × Primer Mix Concentration: 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Loop F / B;
2. Ana ba da shawarar samfuran Nucleic acid don narkar da su ta amfani da ruwan DEPC.
3.Sanya a 65°C na tsawon mintuna 30-45, wanda za'a iya tsawaita daidai gwargwadon canjin launi.
4.Bisa ga ido tsirara, rawaya yana da kyau kuma ja ba shi da kyau.
Bayanan kula
1.Za a iya inganta yawan zafin jiki tsakanin 62 ℃ da 68 ℃ bisa ga yanayin farko.
2.The kunshe-kunshe reagents bai kamata a fallasa zuwa iska na dogon lokaci.
3.Halin canza launin ja da rawaya ya dogara ne akan canjin pH na tsarin amsawa, don Allah kar a yi amfani da maganin ajiya mai ɗauke da Tris nucleic acid, shawarar yin amfani da ddH2Ya adana nucleic acid.
4.Za a gudanar da gwajin a cikin daidaitaccen tsari, ciki har da shirye-shiryen tsarin amsawa, samfurin jiyya da ƙari na samfurin.
5.Ana ba da shawarar shirya tsarin amsawa a cikin tebur mai tsafta kuma ƙara samfuri a cikin hurumin hayaƙi na wasu ɗakuna don guje wa ƙarya.
