AMPPD-Chemiluminescent Substrate
Bayani
AMPPD yana da sassa biyu masu mahimmanci a tsarinsa na kwayoyin halitta.Daya shine dioxygen
zoben tetrad wanda ke haɗa zoben benzene da adamantane, wanda zai iya karya da fitar da photons.Sauran shine kasancewar alkaline phosphatase, wanda ke haifar da kawar da kungiyoyin phosphate kuma ya samar da tsaka-tsaki maras tabbas.Matsakaicin sai ya bazu kuma ya fitar da photons, kuma adadin photon da aka fitar ya yi daidai da tattarawar ALP a cikin maganin, wanda ya dace da tsarin immunoassay na chemiluminescence tare da ALP a matsayin alamar enzyme.
Amfani:
AMPPD wani ruwa ne na kemiluminescent tare da babban hankali, kwanciyar hankali da rabon sigina-zuwa amo, wanda za'a iya amfani dashi don rigakafi na Alkaline Phosphatase (ALP) azaman alama.
Tsarin Sinadarai
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Bayani | Yellow kore bayyananne ruwa |
| Kwanciyar hankali | Ƙimar Iuminescence na 37 ℃ an kiyaye sama da 90% bayan kwanaki 7 na shiryawa a ƙarƙashin kariya ta haske |
| pH darajar | 9.5 ± 0.05 |
| Ƙimar bango | 200-10000 |
| Luminecence darajar high maida hankali CV darajar (idan aka kwatanta da misali, da dauki yanayi sun kasance 150+5μL, da kuma enzyme maida hankali ne 0.0032U/ml) | ≤10% |
Sufuri da ajiya
Sufuri:Na yanayi
Ajiya:Adana a zazzabi na 2-8 ° C, an kiyaye shi sosai daga haske
Nasihar sake gwada Rayuwa:shekara 2
