Cholesterol oxidase (COD/CHOD)
Bayani
Cholesterol oxidase (CHOD) yana haɓaka matakin farko a cikin catabolism na cholesterol.Wasu ƙwayoyin cuta marasa cutarwa, irin su Streptomyces suna iya amfani da cholesterol azaman tushen carbon.Kwayoyin cututtuka, irin su Rhodococcus equi, suna buƙatar CHOD don cutar da macrophage na mai watsa shiri. CHOD yana aiki ne.Cholesterol an fara oxidized zuwa cholest-5-en-3-one a cikin wani mataki na FAD.Cholest-5-en-3-one an ware shi zuwa cholest-4-en3-one. Halin isomerization na iya zama juzu'i a juzu'i.Ayyukan CHOD ya dogara ne akan kaddarorin jiki na membrane wanda aka ɗaure substrate zuwa gare shi.
Ana amfani da CHOD don ƙayyade ƙwayar cholesterol.Shi ne na biyu mafi yawan amfani da enzyme a cikin aikace-aikacen bincike bayan glucose oxidase.CHOD kuma ya sami aikace-aikace a cikin microanalysis na steroids a cikin samfurori na abinci da kuma bambanta 3-ketosteroids daga 3b-hydroxysteroids.Transgenic shuke-shuke da ke bayyana cholesterol oxidase ana binciken a cikin yaki da auduga boll weevil.Hakanan an yi amfani da Cholesterol oxidase azaman bincike na ƙwayoyin cuta don haɓaka tsarin membrane na salula.
Tsarin Sinadarai
Ƙa'idar amsawa
Cholesterol + O2 →△4-Cholesten-3-daya + H2O2
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Bayani | Yellowish amorphous foda, lyophilized |
| Ayyuka | ≥8U/mg |
| Tsarki (SDS-SHAFIN) | ≥90% |
| Solubility (10mg foda / ml) | Share |
| Catalase | ≤0.001% |
| Glucose oxidase | ≤0.01% |
| Cholesterol esterase | ≤0.01% |
| ATPase | ≤0.005% |
Sufuri da ajiya
Sufuri:An aika kasa da -15 ° C
Ajiya:Adana a -25 ~ -15°C(Logon lokaci), 2-8°C (Gajeren lokaci)
An shawarar sake gwadawaRayuwa:shekara 1
