D-Lactate dehydrogenase (LDH)
Bayani
Ana amfani da enzyme don cire pyruvate a cikin ƙaddarar aiki tare da NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Amfani
1.Alkaline phosphatase guda biyu zuwa sunadaran (maganin rigakafi, streptavidin da dai sauransu,) na iya gano takamaiman kwayoyin da aka yi niyya, kuma ana iya amfani da su a cikin ELISA, WB da gano histochemical;
2.Alkaline phosphatase za a iya amfani dashi don dephosphorize 5 '-terminal na DNA ko RNA don hana haɗin kai;
3.Dan ko RNA da ke sama na dephosphorylated ana iya lakafta su ta hanyar phosphates mai lakabin rediyo (ta hanyar T4 poly-nucleotide kinase)
Tsarin Sinadarai
Hanyar amsawa
D-Lactate + NAD+→ Pyruvate +NADH + H+
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Bayani | Farin amorphous foda, lyophilized |
| Ayyuka | ≥200U/mg |
| Tsarki (SDS-SHAFIN) | ≥90% |
| Solubility (10mg foda / ml) | Share |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Sufuri da ajiya
Sufuri:Natsuwa
Ajiya:Ajiye a -20°C (Logon lokaci), 2-8°C (gajeren lokaci)
An shawarar sake gwadawaRayuwa:shekara 2
