Gwajin Glycohemoglobin A1c (HbA1c).
Amfani
● Babban daidaito
● Ƙarfin ƙarfin hana tsangwama
● Kyakkyawan kwanciyar hankali
Tsarin Sinadarai
Aikace-aikace
Gwajin in vitro don ƙididdige ƙimar ƙimar HbA1c a cikin jinin ɗan adam gaba ɗaya akan tsarin photometric.HbA1c samfuri ne na haemoglobin (Hb) wanda ke haifar da jinkirin da ci gaba da amsawar glycation maras enzymatic ƙarƙashin hawan jini.Glucose yana canza haemoglobin musamman a cikin ragowar valine na n-terminal don samar da haemoglobin glycated.A ƙarƙashin yanayin yanayin ilimin lissafi na al'ada, samar da samfuran amsawar glycosylation marasa enzymatic yana daidai da haɓakar masu amsawa.Tunda hargitsin haemoglobin ya kasance da kwanciyar hankali, matakan glycosylation sun dogara ne akan tattarawar glucose kuma suna da alaƙa da tsayin haemoglobin da bayyanar glucose.Don haka, HbA1c alama ce mai kyau ga matsakaicin matakin glucose na jini na watanni 2-3 na ƙarshe.
Ka'ida
A ƙarƙashin aikin protease, an yanke n-terminal na sarkar β a cikin HbA1c kuma an saki dipeptides glycosylated.A cikin martani na farko, ana iya samun maida hankali na Hb ta hanyar auna ɗaukar nauyin 480 nm.A cikin amsa ta biyu, fructosyl peptide oxidase (FPOX) yana aiki akan dipeptides glycosylated don samar da hydrogen peroxide wanda zai iya amsawa tare da wakilai na chromogenic don samar da abin sha a 660nm a gaban peroxidase, to ana iya samun maida hankali na HbA1c ta hanyar auna ɗaukar abun ciki. 660nm ku.Dangane da adadin HbA1c da aka samu da tattarawar Hb, ana iya ƙididdige yawan adadin HbA1c (HbA1c%).
Aiwatar da
Hitachi 7180/7170/7060/7600 Na'urar nazarin halittu ta atomatik, Abbot 16000, OLYMPUS AU640 Na'urar nazarin halittu ta atomatik
Reagents
| Abubuwan da aka gyara | Hankali |
| Reagent 1 (R1) | |
| Buffer mai kyau | 100 mmol/L |
| PRK | 500 KU/L |
| DA-67 | 10 mmol/L |
| Reagents 2 (R2) | |
| Buffer mai kyau | 100 mmol/L |
| Fructosyl peptide oxidase | 50 KU/L |
| Reagent 3 (R3) | |
| Buffer mai kyau | 100 mmol/L |
Sufuri da ajiya
Sufuri:yanayi
Ajiya da Kwanciyar hankali:
Har zuwa ranar karewa da aka nuna akan alamar, lokacin da aka adana ba a buɗe ba a 2-8 ℃ kuma an kiyaye shi daga haske.Da zarar an buɗe, reagents suna da ƙarfi na tsawon kwanaki 28 lokacin da aka sanya su a cikin na'urar tantancewa ko firiji.
Dole ne a guji gurɓata abubuwan da ke cikin reagents.Kada a daskare reagents.
Da zarar narkar da, da calibrator ne barga for 15 kwanaki a 2-8 ℃, da iko ne barga for 7 kwanaki a 2-8 ℃, kada ku daskare.
Rayuwar Shelf:shekara 1
