Hexokinase (HK)
Bayani
Yi amfani da Hexokinase don ƙayyade D-glucose, D-fructose, da D-sorbitol a cikin samfuran bincike na abinci ko nazarin halittu.Hakanan ana amfani da enzyme don tantance wasu saccharide waɗanda ke canzawa zuwa glucose ko fructose, don haka yana da amfani a cikin gwajin glycosides da yawa.
Idan an yi amfani da Hexokinase a hade tare da glucose-6-phosphate dehydrogenase (G6P-DH)* (auna glucose6-phosphate da Hexokinase ya kafa), samfurori bai kamata su kasance na babban adadin phosphate ba kamar yadda G6P-DH ke hana shi ta hanyar phosphate.
Tsarin Sinadarai
Ƙa'idar amsawa
D-Hexose + ATP --mg2+→ D-Hexose-6-phosphate + ADP
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Bayani | Fari zuwa ƙaramin rawaya amorphous foda, lyophilized |
| Ayyuka | ≥30U/mg |
| Tsarki (SDS-SHAFIN) | ≥90% |
| Solubility (10mg foda / ml) | Share |
| Cutar cututtuka | ≤0.01% |
| ATPase | ≤0.03% |
| Phosphoglucose isomerase | ≤0.001% |
| Creatine phosphokinase | ≤0.001% |
| Glucose-6-phosphate dehydrogenase | ≤0.01% |
| NADH/NADPH oxidase | ≤0.01% |
Sufuri da ajiya
Sufuri: Ayanayi
Ajiya:Ajiye a -20°C (Logon lokaci), 2-8°C (gajeren lokaci)
An shawarar sake gwadawaRayuwa:shekara 1
samfurori masu dangantaka
Ku rubuta sakonku anan ku aiko mana
