Kit ɗin bincike na Mataki ɗaya na RT-qPCR
Bayani
Mataki ɗaya qRT-PCR Probe Kit an tsara shi musamman don qPCR wanda ke amfani da RNA kai tsaye (misali kwayar cutar RNA) azaman samfuri.Yin amfani da ƙayyadaddun ƙayyadaddun ƙayyadaddun kwayoyin halitta (GSP), ana iya kammala rubutun juzu'i da qPCR a cikin bututu guda ɗaya, yana rage mahimman hanyoyin bututu da haɗarin kamuwa da cuta.Ana iya kunna shi a 55 ℃ ba tare da shafar inganci da azancin qRT-PCR ba.Haɗa mafi kyawun aikin HiScript III Reverse Transcriptase da Champagne Taq DNA Polymerase na farawa mai zafi, tare da ingantaccen tsarin buffer, ƙwarewar gano matakin Mataki na qRT-PCR Probe Kit na iya kaiwa 0.1 pg na jimlar RNA ko ƙasa da kwafin 10 na samfuran RNA. kuma yana da kwanciyar hankali na thermal mafi girma.Ana ba da Kit ɗin Binciken Mataki ɗaya na qRT-PCR a cikin Babban Mix.Haɗin Mataki na 5 × ɗaya ya ƙunshi ingantaccen buffer da dNTP/dUTP Mix, kuma ya dace da ƙayyadaddun ƙayyadaddun tsarin ganowa dangane da alamun haske mai suna (misali TaqMan).
Tsarin martani
Abubuwan da aka gyara
| Abubuwan da aka gyara | 100rxn | 1,000 rns | 5,000 rxn |
| ddH2O mara kyauta | 2*1 ml | ml 20 | 100 ml |
| 5* mixing mataki daya | 600 μl | 6*1 ml | ml 30 |
| Haɗin enzyme mataki ɗaya | 150 μl | 2*750ml | 7.5ml ku |
| 50* ROX mai magana Dye 1 | 60ml ku | 600 μl | 3*1 ml |
| 50* ROX mai magana Dye 2 | 60ml ku | 600 μl | 3*1 ml |
a.Buffer Mataki ɗaya ya haɗa da dNTP Mix da Mg2+.
b.Enzyme Mix yana ƙunshe da baya
transcriptase, Hot Start Taq DNA polymerase (gyaran antibody) da RNase inhibitor.
c.Ana amfani dashi don gyara kuskuren sinal fluorescene tsakanin rijiyoyi daban-daban.
c.ROX: Kuna buƙatar zaɓar daidaitawa bisa ga ƙirar kayan aikin gwaji.
Aikace-aikace
Gano ƙwayoyin cuta
bincike da bincike na ƙari
Gano cutar dabbobi
Binciken farko na cututtuka na gado
Gano ƙwayoyin cuta na ƙwayoyin cuta a cikin abinci
Shipping da Adana
Sufuri:Fakitin kankara
Yanayin Ajiya:Adana a -30 ~ -15 ℃.
Rayuwar Shief:shekara 1
