Phosphatase Alkaline (ALP)
Bayani
Alkaline Phosphatase an samo shi daga recombinant E. coli iri wanda ke dauke da kwayar halittar TAB5.Enzyme yana haifar da dephosphorylation na 5' da 3' ƙarshen DNA da RNA phosphomonoesters.Har ila yau, yana lalata ribose, da kuma deoxyribonucleoside triphosphates (NTPs da dNTPs).TAB5 Alkaline Phosphatase yana aiki akan 5′ fidda kai, 5′ da tsautsayi da ƙarewa.Ana iya amfani da Phosphatase a aikace-aikacen ilimin halitta da yawa, irin su cloning ko alamar ƙarshen bincike don cire ƙarshen phosphorylated na DNA ko RNA.A cikin gwaje-gwajen cloning, dephosphorylation yana hana DNA ɗin plasmid mai layin layi daga haɗa kai.Hakanan yana iya lalata dNTPs marasa haɗin gwiwa a cikin halayen PCR don shirya samfuri don jerin DNA.Enzyme gaba ɗaya kuma ba a iya jujjuya shi ba ta hanyar dumama a 70 ° C na minti 5, don haka cire phosphatase kafin a haɗa shi ko ƙarshen alamar ba dole ba.
Amfani
1.Alkaline phosphatase guda biyu zuwa sunadaran (maganin rigakafi, streptavidin da dai sauransu,) na iya gano takamaiman kwayoyin da aka yi niyya, kuma ana iya amfani da su a cikin ELISA, WB da gano histochemical;
2.Alkaline phosphatase za a iya amfani dashi don dephosphorize 5 '-terminal na DNA ko RNA don hana haɗin kai;
3.Dan ko RNA da ke sama na dephosphorylated ana iya lakafta su ta hanyar phosphates mai lakabin rediyo (ta hanyar T4 poly-nucleotide kinase)
Tsarin Sinadarai
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Ayyukan Enzyme | 5U/ml |
| Ayyukan Endonuclease | Ba a gano ba |
| Ayyukan Exonuuclease | Ba a gano ba |
| Ayyukan Nicking | Ba a gano ba |
| Ayyukan RNase | Ba a gano ba |
| E.coli DNA | ≤1 kwafi/5U |
| Endotoxin | Gwajin LAL, ≤10EU/mg |
| Tsafta | ≥95% |
Sufuri da ajiya
Sufuri:Natsuwa
Ajiya:Adana a zazzabi na 2-8 ° C
An shawarar sake gwadawaRayuwa:shekara 2
