Peroxidase (Madogararsa Horseradish) Daidaitawa: Hydrogen peroxide oxidoreductase;HRP

Horseradish peroxidase (HRP) an ware shi daga tushen horseradish (Amoracia rusticana) kuma yana cikin rukunin ferroprotopoorphyrin na peroxidases.HRP yana haɗuwa da sauri tare da hydrogen peroxide (H2O2).Sakamakon [HRP-H2O2] hadaddun zai iya oxidize nau'ikan masu ba da gudummawar hydrogen:
Mai Ba da gudummawa + H2O2 → Mai ba da gudummawar Oxidized + 2 H2O
HRP zai oxidize daban-daban substrates (duba Table 1):
• Chromogenic
• Chemiluminescent (kamar luminol ko isoluminol)
• Fluorogenic (irin su tyramine, homovanillic acid, ko 4-hydroxyphenyl acetic acid)
HRP sarkar polypeptide guda ɗaya ce wacce ta ƙunshi gadojin disulfide guda huɗu.HRP shine glycoprotein wanda ya ƙunshi 18% carbohydrate.Abubuwan da ke cikin carbohydrate sun ƙunshi galactose, arabinose, xylose, fucose, mannose, mannosamine, da galactosamine, dangane da takamaiman isozyme.

HRP lakabin da ake amfani da shi sosai don immunoglobulins a cikin aikace-aikacen immunochemistry daban-daban, gami da immunoblotting, immunohistochemistry, da ELISA.Ana iya haɗa HRP zuwa ƙwayoyin rigakafi ta hanyoyi daban-daban, ciki har da glutaraldehyde, periodate oxidation, ta hanyar haɗin disulfide, da kuma ta hanyar amino da thiol masu haɗin kai.HRP ita ce lakabin da aka fi so don ƙwayoyin rigakafi, tun da shi ne mafi ƙanƙanci kuma mafi kwanciyar hankali daga cikin shahararrun alamun enzyme guda uku (peroxidase, β-galactosidase, alkaline phosphatase) kuma glycosylation yana haifar da ƙananan ɗaurin da ba ta dace ba.An buga bita na glutaraldehyde da hanyoyin haɗin lokaci.

Ana kuma amfani da Peroxidase don tantance glucose4 da peroxides a cikin bayani.Littattafai da yawa, 6-24 theses, 25-29 da dissertations 30-46 sun ambaci amfani da P8375 a cikin ka'idojin bincike.