Ultra Nuclease GMP-grade
Lambar Cat: HC2016A
UltraNuclease GMP-grade an bayyana shi kuma an tsarkake shi a cikin Escherichia coli (E.coli) ta hanyar injiniyan kwayoyin halitta kuma an shirya shi a ƙarƙashin yanayin GMP.Yana iya rage danko na sel supernatant da cell lysate a cikin binciken kimiyya, ƙara gina jiki tsarkakewa yadda ya dace da kuma inganta gina jiki aikin bincike.Samfurin kuma zai iya rage ragowar acid nucleic acid zuwa pg-grade, haɓaka aiki da amincin samfuran halittu na aikace-aikace gami da tsarkakewar ƙwayoyin cuta, masana'antar alluran rigakafi, da furotin/polysaccharide na masana'antar harhada magunguna.Bayan haka, ana iya amfani da samfurin don hana cukuɗewar ƙwayoyin halittar jini guda ɗaya (PBMC) a cikin maganin tantanin halitta da haɓakar rigakafin.
Ana ba da UltraNuclease a cikin nau'i na reagent mai haifuwa, wanda aka haɓaka a cikin buffer (20mM Tris-HCL pH 8.0, 2mM MgCl, 20 mM NaCl, 50% glycerin), tare da bayyanar mara launi, ruwa mai haske.An samar da wannan samfurin ta buƙatun tsarin GMP kuma an samar da shi a cikin sigar ruwa.
Abubuwan da aka gyara
UltraNuclease GMP-grade (250 U/μL)
Yanayin Ajiya
Ana jigilar samfurin tare da busasshen ƙanƙara kuma ana iya adana shi a -25 ℃ ~ -15 ° C na shekaru biyu.
Idan an buɗe samfurin kuma an adana shi a 4℃ sama da mako guda, muna ba da shawarar tacewasamfurin don hana gurɓataccen ƙwayoyin cuta.
Ƙayyadaddun bayanai
| Mai watsa shiri Magana | Recombinant E. coli tare da UltraNuclease gene |
| Nauyin Kwayoyin Halitta | 26,5kda |
| soelectric batu | 6.85 |
| Tsafta | ≥99% (SDS-PAGE) |
| Ma'ajiyar Ma'aji | 20mM Tris-HCL pH 8.0, 2mM MgCl, 20 mM NaCl, 50% glycerin |
|
Ma'anar Naúrar | Ma'anar ɗayan Ayyuka ɗaya (U) shine adadin enzyme da aka yi amfani da shi doncanza ƙimar sha na ΔA260 ta 1.0 a cikin mintuna 30 a cikin 2. 625 mltsarin amsawa a 37 ℃ tare da pH na 8.0 (daidai da cikakken narkewa37 μg salmon maniyyi DNA cikin oligonucleotides). |
Umarni
1. Misali Tarin
Kwayoyin maƙwabta: cire matsakaici, wanke sel tare da PBS, kuma cire abin da ke sama.
Kwayoyin dakatarwa: tattara sel ta hanyar centrifugation, wanke sel tare da PBS, centrifuge a 6,000rpm na 10 min, tattara pellet.
Escherichia coli: tattara ƙwayoyin cuta ta hanyar centrifugation, wanke sau ɗaya tare da PBS, centrifuge a 8,000rpm na 5 min, kuma tattara pellet.
2. Misalin Magani
Kula da pellet ɗin tantanin halitta da aka tattara tare da buffer lysis a gwargwadon rabon taro (g) zuwa ƙara (mL)1: (10-20), ko ta hanyar inji ko hanyoyin sinadarai akan kankara ko a zafin jiki (1g na pellet ɗin ta ƙunshi game da
109 sel).
3. Maganin Enzyme
Ƙara 1-5mM MgCl zuwa tsarin amsawa kuma daidaita pH zuwa 8-9.
Ƙara UltraNuclease bisa ga rabo na Raka'a 250 don narke 1 g na pellets tantanin halitta, a sanya shi a 37 ℃ na fiye da minti 30.Da fatan za a koma zuwa fam ɗin "Lokacin Da Aka Shawarci" don zaɓartsawon lokacin jiyya.
4. Mai girma Tarin
Centrifuge a 12,000 rpm na minti 30 kuma tattara abin da ke sama.
Lura: Idan maganin acidic ne ko alkaline, ko ya ƙunshi babban adadin gishiri, kayan wanka, koDenaturants, don Allah ƙara yawan adadin enzyme ko ƙara lokacin jiyya daidai.
Nasihar amsawar yanayions
| Siga | Mafi kyawun Yanayin | Ingantacciyar Yanayi |
| Mg²+Tattaunawa | 1-5 mm | 1-10 mm |
| pH | 8-9 | 6-10 |
| Zazzabi | 37 ℃ | 0-42 ℃ |
| Matsalolin DTT | 0-100 mm | > 0 mM |
| Mercaptoethanol Concentration | 0-100 mm | > 0 mM |
| Monovalent Cation Concentration | 0-20 mm | 0-150 mm |
| Phosphate lon Concentration | 0-10 mm | 0-100 mm |
Nasiha Martani Lokaci (37 ℃, 2 mM Mg²+, pH 8.0)
| Adadin UltraNuclease (Ƙarshe Na Ƙarshe) | Lokacin Amsa |
| 0.25 U/ml | > 10 h |
| 2.5 U/ml | > 4h |
| 25 U/ml | 30 min |
Bayanan kula:
Da fatan za a saka PPE da ake buƙata, irin wannan rigar lab da safar hannu, don tabbatar da lafiyar ku da amincin ku!
