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  • Multiplex Mataki Daya RT-qPCR Premix HCR5141A

Multiplex Mataki Daya RT-qPCR Premix


Saukewa: HCR5141A

Kunshin: 100RXN/1000RXN/10000RXN

Multiplex One Mataki RT-qPCR Premix Kit ɗin PCR ne mai yawa bisa RNA azaman samfuri.

Bayanin Samfura

Bayanin samfur

Bayani

Saukewa: HCR5141A

Multiplex One Mataki RT-qPCR Premix Kit ɗin PCR ne mai yawa bisa RNA azaman samfuri.A cikin gwajin, ana yin jujjuya rubutu da PCR mai ƙididdigewa a cikin bututun amsawa iri ɗaya, sauƙaƙe aikin gwaji da rage haɗarin gurɓata.Za'a iya amfani da ƙira na musamman na buffer da haɗin enzyme a cikin tsarin lyophilized mataki-mataki.Kit ɗin yana amfani da Reverse Transcriptase mai jure zafi don ingantacciyar haɗaɗɗiyar igiyar cDNA ta farko ta amfani da hotstart Taq DNA Polymerase don haɓaka ƙididdigewa.Ya ƙunshi ingantacciyar amsawar amsawa, haɗewar enzymes da sauransu, da abubuwan da ke hana haɓakar PCR marasa takamaiman da haɓaka haɓakar haɓakar halayen qPCR da yawa, yana ba da damar haɓaka ƙimar ƙima mai yawa yayin tabbatar da ingancin haɓakar haɓakawa.

 


  • Na baya:
  • Na gaba:

  • Abubuwan da aka gyara

    Suna

    1. Lyo-Buffer

    2. Lyo-Enzyme Mix

    3. Lyo mai kariya

     

    Yanayin sufuriion

    A: Lyo-buffer da mai karewa: -25 ~ -15 ℃, rayuwar shiryayye shine shekara 1.

    B: Lyo-enzyme Mix, 2-8 ℃, rayuwar shiryayye shine watanni 6.

     

    Umarnin don aiki

    1. Reaction System (Dauki 25μL a matsayin misali)

    Abubuwan da aka gyara

    girma (μL)

    Ƙarshe Tattaunawa

    Lyo-Buffer

    6

    1*

    Lyo-Enzyme Mix

    1

    -

    Lyo-Protectant

    8

    -

     

    Babban Mix (10μM)

    1

    0.1-1 uM

    Binciken Bincike (10μM)

    0.5

    0.05-0.5uM

    Samfuran RNA

    5

    -

    DEPC H2O

    Har zuwa 25

    -

     

    2. Ingantaccen Tsarin Kekuna

    1) Daidaitaccen Tsarin Kekuna

     

    Matakin mayar da martani

    Zazzabi

    Lokaci

    Zagayowar

    1

    Juya rubutun

    50°Ca

    10 min

    1

    2

    denaturation na farko

    95°C

    5 min

    1

     3

     Amplification dauki

    95°C

    dakika 15

     45 hawan keke

    60°Cb

    30 secondsc

     

    2) Ka'idar Keke Mai sauri

     

    Matakin mayar da martani

    Zazzabi

    Lokaci

    Zagayowar

    1

    Juya rubutun

    50°Ca

    2 min

    1

    2

    denaturation na farko

    95°C

    2 dakika

    1

     3

     Amplification dauki

    95°C

    1 dakika

     

    45 hawan keke

    60°Cb

    dakika 13c

    Lura:

    a) Juya baya rubuce-rubuce: Zazzabi na iya zaɓar 42 ° C ko 50 ° C na minti 10-15.

    b) Amplification dauki: Ana daidaita zafin jiki bisa ga ƙimar Tm na abubuwan da aka tsara.

    c)Fluorescence sigina samu: Da fatan za a saita tsarin gwaji bisa ga buƙatun nakayan aiki manual.

     

    Bayanin Fasaha/Kayyadewa 

    Zafafan Farawa

    Gina-in zafi farawa

    Hanyar ganowa

    Gano na farko-bincike

    Hanyar PCR

    Mataki ɗaya RT-qPCR

    Nau'in samfurin

    RNA

     

    Bayanan kula

    1. Wannan samfurin don amfanin bincike ne kawai.

    2. Da fatan za a saka PPE da ake buƙata, irin wannan rigar lab da safar hannu, don tabbatar da lafiyar ku da amincin ku!

    Ku rubuta sakonku anan ku aiko mana