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2 × HiF Taq tare da Jagora Mix HCR2014B Hoton da aka Fitar
  • 2 × HiF Taq da Jagora Mix HCR2014B

2 × HiF Taq da Jagora Mix


Lambar Cat: HCR2014B

Kunshin: 1ml/5ml/25ml

HIF Taq tare da Jagora Mix (Tare da Dye) shiri ne don amfani 2 × premixed bayani wanda ya ƙunshi Plus HIF DNA Polymerase, dNTPs, da ingantaccen buffer.

Bayanin Samfura

Bayanin samfur

Lambar Cat: HCR2014B

HIF Taq tare da Jagora Mix (Tare da Dye) shiri ne don amfani 2 × premixed bayani wanda ya ƙunshi Plus HIF DNA Polymerase, dNTPs, da ingantaccen buffer.Kwayoyin rigakafin monoclonal guda biyu a cikin dakin da zafin jiki wanda ke hana ayyukan polymerase da 3′→ 5′exonuclease ayyukan ana ƙara su zuwa babban mahaɗin don sauƙi da takamaiman takamaiman Hot Start PCR.Ana ƙara haɓakar haɓakar haɓakar maigidan don ba da enzyme tsawon ƙarfin haɓaka juzu'i, tsayin ƙarawa zai iya zama har zuwa 13 kb, enzyme yana da 5′→3′ DNA polymerase aiki da 3′→5′′ Ayyukan exonuclease, amincin sa shine sau 83 na Taq DNA polymerase, wanda shine sau 9 na talakawa DNA polymerase.Ya dace da haɓaka samfuran hadaddun, samfurin haɓakawa shine ƙarshen mara kyau.

2 × HIF Taq tare da Jagora Mix (Tare da Dye) yana da fa'idodi na sauri da sauƙi, babban hankali, ƙayyadaddun ƙaƙƙarfan ƙayyadaddun ƙarfi, kwanciyar hankali mai kyau, da sauransu, tsarin amsawa kawai yana buƙatar ƙara ƙirar ƙira da samfura, kuma ana iya haɓaka su ta biyu- ka'idar mataki, sauƙaƙe matakan gwaji da adana lokaci.Wannan samfurin ya ƙunshi rini mai nuna electrophoresis, kuma samfuran PCR ana iya amfani da su kai tsaye don electrophoresis.Bugu da kari, samfurin kuma ya ƙunshi takamaiman wakili na kariya, ta yadda mahaɗin maigidan zai iya kula da kwanciyar hankali bayan daskarewa da aka maimaita.


  • Na baya:
  • Na gaba:

  • Yanayin Ajiya

    Ya kamata a adana samfuran a -25 ~ -15 ℃ don 1 shekara.

     

    Ƙayyadaddun bayanai

    Ƙayyadaddun samfur

    Babban Mix

    Hankali

    Zafafan Farawa

    Wurin Farawa Mai Kyau

    Tsayawa

    Batsa

    Gudun amsawa

    Mai sauri

    Girman (samfurin ƙarshe)

    Har zuwa 13kb

    Sharuɗɗan sufuri

    Busasshen ƙanƙara

    Nau'in samfur

    Babban amincin PCR premixes

     

    Umarni

    1.Tsarin amsawar PCR

    Abubuwan da aka gyara

    girma (μL)

    Samfurin DNA

    Dace

    Gabatarwa (10 μmol/L)

    2.5

    Reverse Primer (10 μmol/L)

    2.5

    2 × HIF Taq da Jagora Mix

    25

    ddH2O

    zuwa 50

     

    2.An shawarar yin amfani da samfuri daban-daban

    Nau'in samfuri

    Ƙara guntu daga 1kb zuwa 10kb

    Genomic DNA

    50-200 NG

    Plasmid ko Viral DNA

    10 pg-20

    cDNA

    1-2.5 µL (Kada ku wuce 10% na ƙarar amsawar PCR na ƙarshe)

     

    3.Ƙarfafa Ƙarfafawa

    1) Protocol mataki-biyu (samfurin rikitarwa)

    Zagayowar mataki

    Temp.

    Lokaci

    Zagaye

    denaturation na farko

    98 ℃

    3 min

    1

    Denaturation

    98 ℃

    10 seconds

    30-35

    Tsawaita

    68 ℃

    30 dak/kb

    Ƙarshe na ƙarshe

    72 ℃

    5 min

    1

     

    2) Protocol mataki uku (ka'ida ta yau da kullun)

    Zagayowar mataki

    Temp.

    Lokaci

    Zagaye

    denaturation na farko

    98 ℃

    3 min

    1

    Denaturation

    98 ℃

    10 seconds

    30-35

    Annealing

    60 ℃

    dakika 20

    Tsawaita

    72 ℃

    30 dak/kb

    Ƙarshe na ƙarshe

    72 ℃

    5 min

    1

     

    3) Annealing Gradient Protocol (complexity template)

    Zagayowar mataki

    Zazzabi

    Lokaci

    Zagaye

    denaturation na farko

    98 ℃

    3 min

    1

    Denaturation

    98 ℃

    10 dakika

    15 (1℃ raguwa a kowane zagaye)

    Rarraba annealing

    70-55 ℃

    dakika 20

    Tsawaita

    72 ℃

    30 dak/kb

    Denaturation

    98 ℃

    10 dakika

     

    20

    Annealing

    55 ℃

    dakika 20

    Tsawaita

    72 ℃

    30 dak/kb

    Ƙarshe na ƙarshe

    72 ℃

    5 min

    1

     

    Fasaloli a ƙarƙashin ƙa'idar haɓakawa daban-daban

    Protocol

    Mataki Biyu

    Mataki na uku

    Rarraba annealing

    Spec.

    sauri

    matsakaici

    a hankali

    Musamman

    babba

    matsakaici

    babba

    Farashin PCR

    matsakaici

    babba

    matsakaici

    Yawan ganowa

    babba

    matsakaici

    babba

     

    Bayanan kula

    Da fatan za a saka PPE da ake buƙata, irin wannan rigar lab da safar hannu, don tabbatar da lafiyar ku da amincin ku!

     

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