Samfurin Sakin Reagent
Samfurin Sakin Reagent shine don yanayin bincike na POCT na kwayoyin halitta.Don tsarin guda biyu na LAMP na haɓaka kai tsaye da haɓakawa kai tsaye PCR, babu buƙatar cirewar acid nucleic.Za'a iya ƙara girman ɗanyen lysate na samfurin kai tsaye, ana iya gano kwayar da aka yi niyya daidai, ana iya ƙara taƙaita lokacin gano samfurin, wanda yayi daidai da buƙatun aikace-aikacen POCT na ƙwayoyin cuta.Ya dace da swabs na hanci, swabs na makogwaro da sauran nau'in samfurin.Za'a iya amfani da samfuran da aka sarrafa kai tsaye don gano ƙimar PCR ko LAMP na ainihin-lokaci, kuma ana iya samun sakamako iri ɗaya kamar hanyoyin hakar al'ada ba tare da rikitattun ayyukan hakar acid nucleic ba.
Yanayin Ajiya
Sufuri da adanawa a zafin jiki.
Kula da inganci
Ganewar aiki - qPCR mai ƙididdigewa: An haɓaka tsarin Sakin Sakin Sako na 800μl
tare da kwafin 1000 Novel Pseudovirus, samfurin swab na hanci guda ɗaya, wanda ya haifar da irin ƙarfin haɓakawa daƘimar ΔCt tsakanin ± 0.5 Ct.
Tsarin Gwajires
1. Dauki 800 μl Samfurin Sakin Reagent kuma yada maganin lysis cikin bututun samfurin 1.5 ml
2. Ɗauki swab na hanci ko maƙogwaro tare da swab;Tsarin samfurin swab na hanci: ɗauki swab ɗin bakararre a saka shi a cikin hanci, sannu a hankali gaba zuwa zurfin 1.5 cm, a hankali juya sau 4 a kan mucosa na hanci fiye da 15 seconds. , Sa'an nan kuma maimaita wannan aiki a kan sauran kogin hanci tare da wannan swab.Maƙarƙashiya swab samfurin samfurin: ɗauki swab bakararre kuma a hankali, da sauri goge tonsils na pharyngeal da bangon pharyngeal na baya sau 3.
3.Sanya swab nan da nan a cikin bututun samfur.Ya kamata a jujjuya kan swab kuma a haɗe a cikin maganin ajiya na akalla daƙiƙa 30 don tabbatar da cewa samfurin ya ƙare a cikin bututun samfur.
4. Shigarwa a dakin da zafin jiki (20 ~ 25 ℃) don 1min, an kammala shirye-shiryen buffer lysis.
5. Dukansu 25μl tsarin RT-PCR da RT-LAMP sun dace da 10μl adadin samfurin samfuri don gwaje-gwajen ganowa.
Bayanan kula
1. Matsakaicin adadin samfurin lysate kai tsaye wanda ya dace da swab guda ɗaya za'a iya daidaita shi zuwa 400μl, wanda za'a iya daidaita shi bisa ga bukatun gwaji.
2. Da zarar samfurin sarrafa ta samfurin sakewa reagent, ana bada shawara don gudanar da na gaba lokaci na gwajin da wuri-wuri, da tazara jiran lokaci ne zai fi dacewa kasa da 1 hour.
3. pH na samfurin lysate shine acidic, kuma tsarin ganowa yana buƙatar samun wani buffer.Ya dace da mafi yawan PCR, RT-PCR, da LAMP gano hasken walƙiya tare da buffer pH, amma bai dace da gano launi na LAMP ba tare da buffer ba.
