Bst 2.0 DNA Polymerase (Glycerol kyauta, babban yawa)
Bst DNA polymerase V2 an samo shi ne daga Bacillus stearothermophilus DNA Polymerase I, wanda ke da 5'→3' Ayyukan polymerase na DNA da kuma aikin maye gurbin sarkar mai ƙarfi, amma babu 5'→3' ayyukan exonuclease.Bst DNA Polymerase V2 ya dace da matsuguni-matsala, haɓakar haɓakar isothermal LAMP (Maɗaukakin madaidaicin isothermal ƙarawa) da saurin jeri.Wannan Bst DNA polymerase V2 yana da ikon hana ayyukan DNA polymerase a zafin jiki, ta yadda za'a iya sarrafa shi kuma ana iya tsara tsarin amsawa a cikin zafin jiki, yana hana haɓakawa da ba takamaiman takamaiman ba da haɓaka haɓakar halayen, kuma wannan sigar zata iya. zama lyophilized.Bugu da kari, yana da ikon sakin ayyukansa a yanayin zafi mai tsayi, don haka kawar da buƙatar wani matakin kunnawa daban.
Abubuwan da aka gyara
| Bangaren | HC5007A-01 | HC5007A-02 | HC5007A-03 |
| Bst DNA polymerase V2 (Glycerol-free) (32U/μL) | 0.05 ml | 0.25 ml | 2.5 ml |
| 10 × HC Bst V2 Buffer | 1.5 ml | 2 × 1.5 ml | 3 × 10 ml |
| MgSO4 (100mM) | 1.5 ml | 2 × 1.5 ml | 2 × 10 ml |
Aikace-aikace
1.LAMP isothermal haɓakawa
2.Halin motsi na DNA guda ɗaya
3.Babban jerin kwayoyin halittar GC
4.Tsarin DNA na matakin nanogram.
Yanayin Ajiya
Jirgin da ke ƙarƙashin 0 ° C kuma a adana shi a -25 ° C ~ -15 ° C.
Ma'anar Naúrar
An bayyana raka'a ɗaya azaman adadin enzyme wanda ya haɗa 25 nmol na dNTP cikin kayan da ba za a iya narkewa ba a cikin mintuna 30 a 65°C.
Ra'ayin LAMP
| Abubuwan da aka gyara | 25 μLTsari |
| 10 × HC Bst V2 Buffer | 2.5 ml |
| MgSO4 (100mM) | 1.5 μl |
| dNTPs (10mM kowane) | 3.5 ml |
| SYTO™ 16 Green (25×)a | 1.0 μl |
| Haɗin farkob | 6 ml |
| Bst DNA Polymerase V2 (Babu Glycerol) (32 U/ul) | 0.25 ml |
| Samfura | × μL |
| ddH₂O | Har zuwa 25 μl |
Bayanan kula:
1) a.SYTOTM 16 Green (25×): Dangane da buƙatun gwaji, ana iya amfani da sauran rina a madadin;
2) b.Haɗin farko: samu ta hanyar haɗa 20 µM FIP, 20 µM BIP, 2.5 µM F3, 2.5 µM B3, 5 µM LF, 5 µM LB da sauran kundin.
Martani da Hali
1 × HC Bst V2 Buffer, yawan zafin jiki yana tsakanin 60°C da 65°C.
Rashin kunna zafi
80 ° C, 20 min.
