Multiplex Mataki Daya RT-qPCR Premix
Bayani
Saukewa: HCR5141A
Multiplex One Mataki RT-qPCR Premix Kit ɗin PCR ne mai yawa bisa RNA azaman samfuri.A cikin gwajin, ana yin jujjuya rubutu da PCR mai ƙididdigewa a cikin bututun amsawa iri ɗaya, sauƙaƙe aikin gwaji da rage haɗarin gurɓata.Za'a iya amfani da ƙira na musamman na buffer da haɗin enzyme a cikin tsarin lyophilized mataki-mataki.Kit ɗin yana amfani da Reverse Transcriptase mai jure zafi don ingantacciyar haɗaɗɗiyar igiyar cDNA ta farko ta amfani da hotstart Taq DNA Polymerase don haɓaka ƙididdigewa.Ya ƙunshi ingantacciyar amsawar amsawa, haɗewar enzymes da sauransu, da abubuwan da ke hana haɓakar PCR marasa takamaiman da haɓaka haɓakar haɓakar halayen qPCR da yawa, yana ba da damar haɓaka ƙimar ƙima mai yawa yayin tabbatar da ingancin haɓakar haɓakawa.
Abubuwan da aka gyara
Suna |
1. Lyo-Buffer |
2. Lyo-Enzyme Mix |
3. Lyo mai kariya |
Yanayin sufuriion
A: Lyo-buffer da mai karewa: -25 ~ -15 ℃, rayuwar shiryayye shine shekara 1.
B: Lyo-enzyme Mix, 2-8 ℃, rayuwar shiryayye shine watanni 6.
Umarnin don aiki
1. Reaction System (Dauki 25μL a matsayin misali)
Abubuwan da aka gyara | girma (μL) | Ƙarshe Tattaunawa |
Lyo-Buffer | 6 | 1* |
Lyo-Enzyme Mix | 1 | - |
Lyo-Protectant | 8 | - |
Babban Mix (10μM) | 1 | 0.1-1 uM |
Binciken Bincike (10μM) | 0.5 | 0.05-0.5uM |
Samfuran RNA | 5 | - |
DEPC H2O | Har zuwa 25 | - |
2. Ingantaccen Tsarin Kekuna
1) Daidaitaccen Tsarin Kekuna
Matakin mayar da martani | Zazzabi | Lokaci | Zagayowar | |
1 | Juya rubutun | 50°Ca | 10 min | 1 |
2 | denaturation na farko | 95°C | 5 min | 1 |
3 | Amplification dauki | 95°C | dakika 15 | 45 hawan keke |
60°Cb | 30 secondsc |
2) Ka'idar Keke Mai sauri
| Matakin mayar da martani | Zazzabi | Lokaci | Zagayowar |
1 | Juya rubutun | 50°Ca | 2 min | 1 |
2 | denaturation na farko | 95°C | 2 dakika | 1 |
3 | Amplification dauki | 95°C | 1 dakika |
45 hawan keke |
60°Cb | dakika 13c |
Lura:
a) Juya baya rubuce-rubuce: Zazzabi na iya zaɓar 42 ° C ko 50 ° C na minti 10-15.
b) Amplification dauki: Ana daidaita zafin jiki bisa ga ƙimar Tm na abubuwan da aka tsara.
c)Fluorescence sigina samu: Da fatan za a saita tsarin gwaji bisa ga buƙatun nakayan aiki manual.
Bayanin Fasaha/Kayyadewa
Zafafan Farawa | Gina-in zafi farawa |
Hanyar ganowa | Gano na farko-bincike |
Hanyar PCR | Mataki ɗaya RT-qPCR |
Nau'in samfurin | RNA |
Bayanan kula
1. Wannan samfurin don amfanin bincike ne kawai.
2. Da fatan za a saka PPE da ake buƙata, irin wannan rigar lab da safar hannu, don tabbatar da lafiyar ku da amincin ku!