Ribonuclease A (RNase A)

Ribonuclease A (RNase A) wani polypeptide ne mai madauri guda ɗaya mai ɗauke da 4 disulfide bonds tare da nauyin kwayoyin halitta na kusan 13.7 kDa.RNase A shine endoribonuclease wanda ke lalata RNA mai madauri ɗaya musamman a ragowar C da U.Musamman, cleavage ya gane haɗin phosphodiester da aka kafa ta 5'-ribose na nucleotide da ƙungiyar phosphate akan 3'-ribose na pyrimidine nucleotide na kusa, don haka 2', 3' - Cyclic phosphates suna hydrolyzed zuwa daidai. 3'-nucleoside phosphates (misali, pG-pG-pC-pA-pG RNase A ce ta raba don samar da pG-pG-pCp da A-PG).RNase A shine ya fi aiki a cikin ƙwanƙwasa RNA mai madauri guda ɗaya kuma yana aiki a cikin yanayi daban-daban: a ƙarancin gishiri mai yawa (0 zuwa 100 mM NaCl), ana iya amfani dashi don manne RNA mai ɗaci ɗaya, RNA mai madauri biyu, da RNA strands a cikin RNA-DNA hybrids.yayin da yake cikin yawan gishiri mai yawa (≥0.3 M), RNase A na iya musamman manne RNA mai madauri ɗaya.