Mataki Daya Mai Saurin RT-qPCR Probe Premix-UNG
Saukewa: HCR5143A
Kit ɗin Binciken Mataki ɗaya na RT-qPCR (DON FAST) kayan bincike ne na tushen RT-qPCR mai saurin ganowa wanda ya dace da PCR-plex ko multiplex mai ƙididdigewa ta amfani da RNA azaman samfuri (kamar ƙwayar cuta ta RNA).Wannan samfurin yana amfani da sabon ƙarni na Taq DNA Polymerase da aka gyaggyara antibody da kuma sadaukarwar mataki ɗaya na Reverse Transcriptase, tare da ingantaccen buffer don haɓakawa cikin sauri, wanda ke da saurin haɓakawa da sauri, ingantaccen haɓakawa da ƙayyadaddun bayanai.Yana goyan bayan haɓaka ma'auni a cikin duka guda-plex da multiplex na ƙananan samfurori masu girma a cikin ɗan gajeren lokaci.
Abubuwan da aka gyara
1.5×RT-qPCR buffer (U+)
2. Haɗin Enzyme (U+)
Bayanan kula:
a.5×RT-qPCR buffer (U+) ya ƙunshi dNTP da Mg2+;
b.Haɗin Enzyme (U+) ya haɗa da reverse transcriptase, Hot Start Taq DNA polymerase, RNase inhibitor da UDG;
c.Yi amfani da nasihu marasa kyauta na RNase, bututun EP, da sauransu.
Kafin amfani, haɗa sosai da 5×RT-qPCR buffer (U+).Idan akwai wani hazo bayan narke, jira buffer ya koma dakin zafin jiki, gauraya kuma ya narke, sannan a yi amfani da su akai-akai.
Yanayin Ajiya
Ana jigilar samfurin tare da busassun ƙanƙara kuma ana iya adana shi a -25 ~ -15 ℃ na shekara 1.
Umarni
1. Amsa Tsari
| Abubuwan da aka gyara | girma (20 μL amsa) |
| 2 ×RT-qPCR buffer | 4 μl |
| Haɗin Enzyme (U+) | 0.8 ml |
| Gabatarwa | 0.1 ~ 1.0 μM |
| Juya Farko | 0.1 ~ 1.0 μM |
| TaqMan Probe | 0.05 ~ 0.25μM |
| Samfura | X μL |
| RNase-Ruwa | har zuwa 25 μl |
Bayanan kula: Girman amsa shine 10-50μL.
2. Ka'idar Keke (Standar)
| Zagayowar mataki | Temp. | Lokaci | Zagaye |
| Juya Rubutu | 55 ℃ | 10 min | 1 |
| Farkon Denaturation | 95 ℃ | 30 seconds | 1 |
| Denaturation | 95 ℃ | 10 dakika | 45 |
| Annealing/Extension | 60 ℃ | 30 seconds |
| Ka'idar Keke (Mai sauri) Zagayowar mataki |
Temp. |
Lokaci |
Zagaye |
| Juya Rubutu | 55 ℃ | 5 min | 1 |
| Farkon Denaturation | 95 ℃ | 5 s ku | 1 |
| Denaturation | 95 ℃ | 3 s ku | 43 |
| Annealing/Extension | 60 ℃ | 10 s ku |
Bayanan kula:
a.Zazzabi na juye juye yana tsakanin 50 ℃ zuwa 60 ℃, ƙara yawan zafin jiki yana taimakawa haɓaka hadaddun sifofi da samfuran abun ciki na CG mai girma;
b.Ana buƙatar daidaita madaidaicin zafin jiki na annealing dangane da ƙimar Tm na firamare, kuma zaɓi mafi ƙarancin lokaci don tarin siginar walƙiya dangane da kayan aikin PCR na Real Time.
Bayanan kula
Da fatan za a saka PPE da ake buƙata, irin wannan rigar lab da safar hannu, don tabbatar da lafiyar ku da amincin ku!
