Babban Haɓaka T7 RNA Poymerase
Bayani
T7 RNA Polymerase ya keɓe daga E.coli yana ɗauke da plasmid wanda ya ƙunshi ƙwayar T7 RNA Polymerase.T7 RNA Polymerase shine RNA polymerase mai dogaro da DNA wanda ke da takamaiman takamaiman masu tallata T7 phage.
Tsarin Sinadarai
Ma'anar Naúrar
An ayyana raka'a ɗaya azaman adadin enzyme wanda zai haɗa 1 nmol ATP cikin kayan acid-insoluble a cikin jimlar amsawar 50 μl a cikin awa 1 a 37°C a cikin 1X RNA Polymerase Reaction Buffer.
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Protein Tsabta | ≥95% |
| Nickase | Babu wanda aka gano |
| Rnase | Babu wanda aka gano |
| Endonuclease | Babu wanda aka gano |
| Exonuclease | Babu wanda aka gano |
| Ƙimar Mai haɓakawa | Samfurin da ba na musamman ≤1.5% |
| E.coli gDNA | ≤0.1 shafi/50U |
| Endotoxin | Gwajin LAL, ≤10EU/mg |
Sufuri da ajiya
Sufuri:Busasshen ƙanƙara
Ajiya:Ya kamata a adana duk abubuwan da aka gyara a -25 ~ -15 ° C
Nasihar sake gwada Rayuwa:shekara 2
samfurori masu dangantaka
Ku rubuta sakonku anan ku aiko mana
