Kit ɗin bincike na Mataki ɗaya na RT-qPCR
Bayani
Mataki ɗaya qRT-PCR Probe Kit an tsara shi musamman don qPCR wanda ke amfani da RNA kai tsaye (misali kwayar cutar RNA) azaman samfuri.Yin amfani da ƙayyadaddun ƙayyadaddun ƙayyadaddun kwayoyin halitta (GSP), ana iya kammala rubutun juzu'i da qPCR a cikin bututu guda ɗaya, yana rage mahimman hanyoyin bututu da haɗarin kamuwa da cuta.Ana iya kunna shi a 55 ℃ ba tare da shafar inganci da azancin qRT-PCR ba.Ana ba da Kit ɗin Binciken Mataki ɗaya na qRT-PCR a cikin Babban Mix.Haɗin Mataki na 5 × ɗaya ya ƙunshi ingantaccen buffer da dNTP/dUTP Mix, kuma ya dace da ƙayyadaddun ƙayyadaddun tsarin ganowa dangane da alamun haske mai suna (misali TaqMan).
Ka'idojin asali na RT-qPCR
Ƙayyadewa
| Kayan Gwaji | Ƙayyadaddun bayanai | Sakamako |
| (SDS PAGE) Tsaftar Hannun Enzyme (SDS PAGE) | ≥95% | Wuce |
| Ayyukan Endonuclease | Ba a gano ba | Wuce |
| Ayyukan Exodulease | Ba a gano ba | Wuce |
| Ayyukan Rnase | Ba a gano ba | Wuce |
| Ragowar E.coli DNA | 1 kwafi/60 | Wuce |
| Tsare-tsare-Tsarin Aikin Assay | 90%≤110% | Wuce |
Abubuwan da aka gyara
| Abubuwan da aka gyara | 100rxn | 1,000 rns | 5,000 rxn |
| ddH2O mara kyauta | 2*1 ml | ml 20 | 100 ml |
| 5* mixing mataki daya | 600 μl | 6*1 ml | ml 30 |
| Haɗin enzyme mataki ɗaya | 150 μl | 2*750ml | 7.5ml ku |
| 50* ROX mai magana Dye 1 | 60ml ku | 600 μl | 3*1 ml |
| 50* ROX mai magana Dye 2 | 60ml ku | 600 μl | 3*1 ml |
a.Buffer Mataki ɗaya ya haɗa da dNTP Mix da Mg2+.
b.Enzyme Mix yana ƙunshe da baya
transcriptase, Hot Start Taq DNA polymerase (gyaran antibody) da RNase inhibitor.
c.Ana amfani dashi don gyara kuskuren sinal fluorescene tsakanin rijiyoyi daban-daban.
d.ROX: Kuna buƙatar zaɓar daidaitawa bisa ga ƙirar kayan aikin gwaji.
Aikace-aikace
Gano QPCR
Shipping da Adana
Jirgin ruwa:Fakitin kankara
Yanayin Ajiya:Adana a -20 ℃.
Rayuwar Shief:watanni 18
