Poly A Carrier RNA
Saukewa: HC4001A
Poly A, polyadenylate, cakuda ne na 100 ~ 10000 polyadenylates, wanda aka sanya shi ta hanyar polynucleotide phosphorylase a cikin vitro.A cikin vivo, ana ƙara poly (a) zuwa 3-terminal na mRNA ta enzyme don inganta kwanciyar hankali na mRNA.A cikin aikace-aikacen hakar acid nucleic, ƙara poly A zuwa lysate ko dauri bayani zai iya inganta yawan amfanin DNA da RNA.Hanyarna poly A inganta yawan amfanin ƙasa na nucleic acid ne kamar haka:
1. Cikakkun lamba tare da tallan abubuwan da ke sama.Yawancin abubuwan polypropylene suna da wutar lantarki a tsaye a saman, wanda zai lalata acid nucleic.Mai ɗaukar RNA na iya cika waɗannanadsorption effects da kuma rage asarar manufa nucleic acid.
2. Inactivate trace nucleases: Akwai daban-daban nucleases a nazarin halittu samfurori da kumamuhalli.Poly A na iya kashe ƙwayoyin ƙwayoyin cuta a cikin matakan cirewa ko kiyayewa zuwainganta yawan amfanin ƙasa da kwanciyar hankali na acid nucleic manufa.
3. Kwatantawa: A cikin nucleic acid tsarkakewa mataki na barasa matsakanci hazo ko dauri, poly A na iya daidaitawa tare da manufa nucleic acid ko samar da polymer barbashi zuwainganta farfadowa.
Yanayin ajiya
-20 ~ 8 ℃, bushe ajiya, dogon lokacin ajiya ya kamata a sanya a -20 ℃
Ƙayyadaddun bayanai
| Lambar CAS | 26763-19-9 |
| Bayyanar | Farin lyophilized foda |
| Tsafta | 99% |
| Nauyin Kwayoyin Halitta | 700-3500 KDa |
Hanyar amfani
Ɗauki adadin da ya dace na lyophilized foda, ƙara ruwan magani na DEPC ko maganin gishiri na guanidine zuwaNarkar da shi cikin 0.1-1ug/ul, sa'an nan kuma sub-pack shi da kuma adana shi a -20 ° C.
Aikace-aikace
1.Virus DNA / RNA hakar: ƙara 1-5ug Carrier RNA zuwa lysate zai iya inganta yawan amfanin ƙasa na RNA / DNA, daidaita maƙasudin nucleic acid da kuma guje wa lalatawar acid mai tsabta a lokacin ajiya.
2. A cikin micro DNA/RNA hakar ta hanyar membrane ginshiƙi (<1ug), ƙara mai ɗaukar RNA zuwa 1-5ug yana da amfani don inganta yawan amfanin ƙasa na nucleic acid.
3. A cikin barasa mai tsaka-tsakin hazo na acid nucleic da matakin maida hankali, ƙari na 1-2ug mai ɗaukar RNA yana taimakawa don haɓaka dawo da ɗan gajeren sashi na RNA.
4. A cikin bincike mai ƙididdigewa PCR bayani na amsawa, ƙara 10-100ng mai ɗaukar RNA zuwa maganin amsawa yana taimakawa wajen haɓaka hankali da rage CTdaraja.
