Superstart qPCR Premix da-UNG

Saukewa: HCB5071E

Superstart qPCR Premix Plus-UNG shine ƙwararren reagent wanda aka tsara don ƙimar PCR na Real Time da halayen ƙididdigewa ta amfani da gano tushen bincike, wanda aka haɓaka musamman don hanyoyin lyophilization.Yana ƙunshe da wani labari mai zafi-farawa enzyme Hotstart Taq plus (DG), wanda ke da aikin Taq enzyme wanda aka hatimce shi a cikin zafin jiki, yadda ya kamata ya hana ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun yanayin zafi ke haifarwa. ƙayyadaddun halayen haɓakawa.Wannan reagent yana amfani da ingantaccen buffer na qPCR da kuma tsarin hana cutar UNG/dUTP don cimma saurin farawa mai zafi, yana haɓaka inganci da azancin halayen qPCR.Yana iya samun ingantattun madaidaitan layukan da ke cikin kewayon wuraren ƙididdigewa da yin ƙididdige ƙididdigewa daidai, yadda ya kamata ya hana haɓaka ingantaccen ingancin ƙarya wanda ya haifar da ragowar samfuran PCR ko gurɓataccen iska.Wannan reagent ya dace da yawancin kayan aikin PCR masu kyalli daga masana'antun kamar Applied Biosystems, Eppendorf, Bio-Rad da Roche da sauransu, kuma yana nuna kyakkyawan kwanciyar hankali a cikin sigar lyophilized.

2. Kwafin adadin kwayoyin halittar da aka yi niyya da ke cikin nau'ikan samfuri daban-daban sun bambanta;idan ya cancanta, za a iya yin dilution gradient don ƙayyade adadin ƙarin samfuri mafi kyau.

3. Wannan tsarin zai iya zama lyophilized;Lokacin da abokan ciniki ke amfani da wannan tsarin ba tare da buƙatun bushewa ba, 4 × lyoprotectant za a iya zaɓin ƙarawa; idan akwai samfuran busassun busassun da ake buƙata, a lokacin ingantaccen aikin samfuran ruwa, dole ne ƙara 4 × lyoprotectant don tabbatar da daidaito tare da abubuwan haɗin tsarin lyophilized. da tasiri.

2. Ƙara samfurin nucleic acid zuwa foda na lyophilized kuma ƙara ruwa har zuwa 25µL;

3. Mix da kyau ta hanyar centrifugation kuma gudu akan na'ura.

 Sarrafa inganci:

1. Gwajin aiki: hankali, ƙayyadaddun ƙayyadaddun bayanai, sake fasalin qPCR.

2. Babu aikin nuclease na waje, babu gurɓataccen endo/exonuclease.

Bayanin Fasaha:

1. Superstart qPCR Premix plus-UNG yana amfani da wani sabon labari mai zafi-farawa enzyme wanda ke ba da damar farawa mai sauri a cikin mintuna 1 ~ 5;ta hanyar ƙirar buffer na musamman ya dace da multix fluorescent quantitative PCR halayen.

2. Yana da mafi girma ƙayyadaddun abin da muhimmanci inganta ji na ƙwarai da haske mai yawa PCR iyaka ganewa, yin ƙarawa masu lankwasa normalization, kyalli darajar samu bayyanannen ci gaba a low taro shaci, dace a matsayin high ji mai kyalli kyalli na PCR gano reagents.

3. Don masu farawa tare da ƙananan zafin jiki na annealing ko fiye da 200bp gutsuttsura, ana ba da shawarar hanyar 3-mataki.

4. Amfanin amfani da dUTP da hankali ga UNG enzyme sun bambanta don nau'in nau'in nau'in manufa daban-daban, don haka idan amfani da tsarin UNG ya haifar da raguwa a cikin ganewar ganewa, ya kamata a daidaita tsarin amsawa da ingantawa.Idan ana buƙatar tallafin fasaha don Allah a tuntuɓi kamfaninmu.

5. Yi amfani da wuraren da aka keɓe da pipettes kafin da bayan haɓakawa, sanya safar hannu yayin aiki kuma maye gurbin su akai-akai;kar a buɗe bututun dauki bayan kammala PCR don rage gurɓatar samfuran samfuran PCR.