Recombinant Deoxyribonuclease I (DNase I, RNase-free)
Bayani
Recombinant Deoxyribonuclease I, wani endonuclease ne wanda zai iya narkar da DNA mai madauri guda ɗaya ko biyu.Yana hydrolyzes phosphodiester bond don samar da mono- da oligodeoxynucleotides dauke da 5'-phosphate kungiyoyin da 3'-OH kungiyoyin.Mafi kyawun kewayon pH mai aiki na DNAse I shine 7-8.Ayyukan DNase I ya dogara da Ca2 + kuma ana iya kunna shi ta hanyar ions na ƙarfe na divalent kamar Co2+, Mn2+, Zn2+, da dai sauransu.yayin da yake gaban Mn2+, DNase I na iya yanke DNA mai ɗaure biyu a wuri ɗaya, samar da ƙarewa mara kyau ko 1-2 nuclei Sticky ƙare tare da overhangs nucleotide.An samo wannan enzyme daga recombinant E. coli damuwa, ba ya ƙunshi RNase, kuma ana iya amfani dashi don sarrafa samfuran RNA daban-daban.
Tsarin sinadaran
Aikace-aikace
RNA hakar: shirya RNA ba tare da DNA ba;
Bayan rubutun in vitro tare da RNA polymerase, kamar T7 RNA Polymerase (Cat # 10618), ana amfani da shi don cire samfurin DNA;
Shirya RNA mara DNA kafin RT-PCR da RT-qPCR;
An yi amfani da shi tare da DNA Polymerase I (Cat#12903) don yiwa DNA lakabi ta fassarar nick;
An yi amfani da shi a cikin nazarin sawun ƙafa don nazarin hulɗar DNA-protein;
Ƙirƙirar ɗakin karatu na guntu bazuwar;
A cikin gano TUNEL na apoptosis, an yi amfani da DNA ɗin genomic juzu'i azaman iko mai inganci.
Shipping da Adana
Sufuri:Fakitin kankara
Yanayin Ajiya:Adana a cikin -20 ℃
Rayuwar Shelf:shekaru 2
Bayanan kula:
1.Enzymes ya kamata a adana a cikin akwati na kankara ko a kan kankara wanka lokacin amfani, kuma ya kamata a adana a -20 ° C nan da nan bayan amfani.
2.Don amincin ku da lafiyar ku, da fatan za a sa kayan kariya na sirri (PPE), kamar sutturar dakin gwaje-gwaje da safofin hannu masu zubarwa, lokacin aiki da wannan samfur.
