RT-LAMP Mai launi Mai launi Mix HCB5204A
Wannan samfurin yana ƙunshe da buffer, RT-Enzymes Mix (Bst DNA polymerase and heat-resistant reverse transcriptase), lyophilized Protectors da chromogenic abubuwan rini.Don amfani kawai, yi amfani da Buffer kawai, ana gauraye enzyme da furotin kuma an ƙara su zuwa samfuri;ƙara lyophilized kariya zai iya zama madaidaiciya.An haɗa shi da lyophilizer da lyophilized, kuma kawai abubuwan da aka haɗa da samfurori da aka ƙara lokacin amfani da su.Wannan kit ɗin yana ba da sauri, bayyananniyar gano gani na haɓakawa, wanda aka nuna mummunan amsa a cikin ja kuma ana nuna sakamako mai kyau ta canji zuwa rawaya.
Bangaren
| Bangaren | Saukewa: HCB5204A-01 | Saukewa: HCB5204A-02 | Saukewa: HCB5204A-03 |
| Matsakaicin Matsakaicin Amplification Buffer (tare da rini) | 0.96 ml | 4.80ml × 2 | 9.60 ml × 10 |
| RT-Enzymes Mix | 270 ml | 2.70 ml | 2.70 ml × 10 |
| Lyophilized kariya | 0.96 ml × 2 | 9.60ml × 2 | 9.60 ml × 20 |
Aikace-aikace
Don DNA ko RNA isothermal amplification.
Yanayin Ajiya
Ana jigilar shi tare da busassun kankara, an adana shi a -25 ~ -15 ℃.Guji daskare-narke akai-akai, samfurin yana aiki har tsawon watanni 12.
Yarjejeniya
1.Narke abin da za a yi amfani da shi a zafin jiki.Vortex a taƙaice ko jujjuya bututu sau da yawa don haɗawa sosai, sa'an nan kuma a hankali don tattara ruwan zuwa kasan bututu.
2.Shiri na dauki tsarin.Wannan reagent za a iya shirya a biyu dauki tsarin, ruwa dauki mix da lyophilized tsarin mix.
1) Shirya ruwa dauki mix
| Bangaren | Ƙarar |
| Matsakaicin Matsakaicin Amplification Buffer (tare da rini) | 10 μl |
| RT-Enzymes Mix | 2.8 ml |
| 10 × Farko Mixa | 5 μl |
| Samfuran DNA/RNA b | × μL |
| Ruwa marar Nukiliya | Har zuwa 50 μl |
2) Lyophilization tsarin Mix
① Shirya lyophilized mix
| Bangaren | Ƙarar |
| Matsakaicin Matsakaicin Amplification Buffer (tare da rini) | 10 μl |
| Lyophilized kariya | 20 μl |
| RT-Enzymes Mix | 2.8 ml |
| Ruwa marar Nukiliya | Har zuwa 50 μl |
② Lyophilization: An shirya Mix Mix a cikin tsarin 50μL
③ Shirya haɗin kai
| Bangaren | Ƙarar |
| Lyophilized Mix | guda 1 |
| 10 × Farko Mixa | 5 μl |
| Samfuran DNA/RNA b | × μL |
| Ruwa marar Nukiliya | Har zuwa 50 μl |
Bayanan kula:
1) a.10 × Primer Mix : 16 μM FIP / BIP, 2 μM F3 / B3, 4 μM Madauki F / B;
2) b.Ana ba da shawarar DEPC (mai narkewar ruwa) don templ na acid nucleic.
1.Sanya a 65°C na tsawon mintuna 30-45, wanda za'a iya tsawaita daidai gwargwadon canjin launi.
2.Bisa ga ido tsirara, rawaya yana da kyau kuma ja ba shi da kyau.
Bayanan kula
1.Gishiri na iya fitowa a kasan buffer, vortex a taƙaice ko jujjuya bututu sau da yawa don haɗuwa sosai a cikin ɗaki.
2.Za'a iya inganta yanayin zafin jiki tsakanin 62 ℃ da 68 ℃ bisa ga yanayin firam.
3.The kunshe-kunshe reagents bai kamata a fallasa zuwa iska na dogon lokaci.
4.Halin canza launin ja da rawaya ya dogara da canjin pH na tsarin amsawa, don Allah kar a yi amfani da maganin adana abun ciki na Tris nucleic acid, shawarar yin amfani da ddH2Ya adana nucleic acid;
5.Gwajin ya kamata a daidaita shi, ciki har da shirye-shiryen tsarin amsawa, lyophilization, da samfurin aiki da samfurin ƙarawa;
6.Don guje wa gurɓatawa, ana ba da shawarar shirya tsarin amsawa a cikin benci mai tsafta, a cikin wasu Ƙara samfuri zuwa murfin hayaƙi na ɗakin don guje wa tsangwama mai kyau na ƙarya.
