T4 RNA ligase
Bayani
Wannan samfurin ligase T4 RNA ne mai dogaro ATP (T4 RNA Ligase I) wanda E. coli ya sake bayyanawa.Wannan enzyme yana haifar da samuwar haɗin phosphodiester tsakanin oligonucleotides, RNA-stranded guda ɗaya da DNA intermolecular / intra-molecular 5'-PO4 da 3'-OH.
Ma'anar Naúrar
An bayyana raka'a ɗaya azaman adadin enzyme da ake buƙata don canza 1 nanomole na 5'-[³²P] rA16 zuwa sigar juriyar phosphatase cikin mintuna 30 a 37°C.
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Ayyukan Enzyme | 10 U/μL |
| Ayyukan furotin | ≥ 95% |
| Nickase | Ba a iya ganowa |
| Ayyukan RNase | Ba a iya ganowa |
| Ecoli gDNA | ≤1 kwafi/50U |
| Endotoxin | Gwajin LCL, ≤10EU/mg |
Sufuri da ajiya
Sufuri:Busasshen ƙanƙara
Ajiya:Ajiye a -25 ~ -15°C (kauce wa maimaita daskarewa da narkewa)
Nasihar sake gwada Rayuwa:shekara 2
samfurori masu dangantaka
Ku rubuta sakonku anan ku aiko mana
