BsaI Ƙuntatawa Endonuclease
Bayani
BsaI ƙuntatawa enzyme shine nau'in ƙuntatawa na II na endonuclease wanda aka samo daga furotin na sake haɗawa ta hanyar BspQI gene a cikin Bacillus sphaericus wanda E.coli ya bayyana.Ana amfani da ƙuntatawa endonucleases a ko'ina a cikin taswirar kwayoyin halitta, cloning da sauran fannoni.Wannan enzyme da sauri ya yanke DNA don ingantaccen layin layi na Bsa I , ƙuntatawa na IIs endonuclease ƙuntatawa endonuclease, an samo shi daga nau'in E. coli mai sake haɗawa wanda ke ɗauke da cloned da gyaran BsaI gene daga Bacillus stearothermophilus.waɗanda ke da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin enzymes na asali da rage ayyukan tauraro.Jerin fitattun sa da wuraren tsagewa sune kamar haka:
5'······GGTCTC(N)·········3'
3'······CCAGAG(NNNN)·······5'
Tsarin Sinadarai
Siffofin samfur
• Ƙuntataccen endonuclease yana da babban aikin enzymatic kuma yana kammala aikin narkewa da sauri.
• Ƙananan ayyukan endonuclease marasa ƙayyadaddun ƙayyadaddun don tabbatar da ainihin yanke "scalpel".
• Babu tsarin BSA, rage gurɓataccen yanayin zafi.
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Ayyukan Enzyme | 20 U/µL |
| Tsarki (SDS-SHAFIN) | ≥95% |
| Rnase | Babu wanda aka gano |
| Ayyukan DNA marasa Takamaiman | Ba a gano ba |
| Ayyukan Tauraro | Ba a gano ba |
| ligation da Ragewa | Bayan narkewa, ana iya haɗa gutsuttsuran DNA.Kuma ana iya yanke waɗannan gutsuttsuran ligated tare da Bsal. |
| Endotoxins (LAL-gwajin) | ≤10 EU/mg |
| E.Coli DNA | ≤1 Kwafi/2U |
Kula da inganci
Tsabta ≥ 95%, Babu DNase, RNase aiki, Mai watsa shiri DNA ragowar ≤ 100pg / MG, mai masaukin furotin ≤ -50ppm, ragowar endotoxin ≤ 10EU / MG, Babu aikin protease, bakararre, kyauta daga mycoplasma
Aikace-aikace
Narke plasmid don shirya guntun DNA mai layi a ƙarshen Poly (A/T/C/G);
Linearize samfurin plasmid kafin rubutun in vitro;
Ƙuntataccen narkewa;
Ma'anar Naúrar
An bayyana raka'a ɗaya azaman adadin enzyme da ake buƙata don narkar da 1 μg na DNA na ciki a cikin awa 1 a 37 ° C a cikin jimlar amsawar 50 µL.
Sufuri da ajiya
Sufuri:Ana aikawa a ƙasa da -15 ° C
Ajiya:Adana a -25 ~ -15 ° C
Nasihar sake gwada Rayuwa:shekara 1
