Ƙuntatawa BspQI Endonuclease
Bayani
Ana iya sake bayyana BspQI a cikin E. coli wanda zai iya gane takamaiman rukunin yanar gizo kuma ana samarwa a ƙarƙashin ƙa'idodin GMP.Ana amfani dashi sosai a cikin taswirar kwayoyin halitta, cloning da sauran fannoni.Wannan enzyme da sauri yana yanke DNA don ingantaccen layin layi.
5'······GGTCTC(N) 1········3'
3'······CCAGAG(N) 4········5'
Tsarin Sinadarai
Ma'anar Naúrar
An bayyana raka'a ɗaya azaman adadin enzyme da ake buƙata don narkar da 1 μg na λ DNA a cikin awa 1 a 50 ° C a cikin jimlar amsawar 50 µl.
Siffofin Samfur
• Ƙuntataccen endonuclease yana da babban aikin enzymatic kuma yana kammala narkewa cikin sauri.
• Ƙananan ayyukan endonuclease marasa ƙayyadaddun ƙayyadaddun don tabbatar da ainihin yanke "scalpel".
• Babu tsarin BSA, rage gurɓataccen yanayin zafi.
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Protein Tsabta | 98% |
| Ragowar Protease | Babu wanda aka gano |
| Haɗi da yanke ganowa | Ingantaccen narkewa -95% |
| DNA | Babu wanda aka gano |
| Nicaknase | Babu wanda aka gano |
Sufuri da ajiya
Sufuri:An aika a ƙarƙashin 0 ° C
Ajiya:Adana a -20 ° C
Nasihar sake gwada Rayuwa:2 shekara
