Recombinant Trypsin
Amfani
1.Babu asalin dabba
2.Tsarki mai girma
3.Better sarrafa tsari
4.Stable inganci
Bayani
Trypsin musamman yana raba C-terminal peptide bonds na lysine da arginine, wanda zai iya lalata ƙwayoyin daurin ƙwayoyin cuta.Jerin amino acid na recombinant trypsin da Hzymes Biotech ke samarwa yayi daidai da na porcine wanda aka samo daga trypsin na pancreatic, kuma ana yin shi ta hanyar recombinant Escherichia coli magana.Yana iya maye gurbin na al'ada hakar trypsin ga cell narkewa a cikin alurar riga kafi, kara Kwayoyin, rigakafi cell far, miyagun ƙwayoyi screening, antibodies da sauran filayen.Aprotinin, mai hana trypsin waken soya, da dai sauransu na iya hana ayyukan trypsin musamman.
Recombinant trypsin yana da kaddarorin iri ɗaya kamar yadda ake fitar da trypsin ta halitta.An ƙera wannan samfurin a cikin wani bita mai daraja ta GMP.Ba a yi amfani da albarkatun asalin dabba ba a cikin fermentation, tsarkakewa da sinadaran ƙarshe.A lokaci guda kuma, ana tabbatar da ayyukan samarwa da sarrafa inganci, wanda ke haifar da babban matakin daidaito tsakanin batches.
Tsarin Sinadarai
Ma'anar Naúrar
25 ℃, pH 7.6, 3.2ml dauki bayani (1cm haske hanya), daya trypsin naúrar (U) aka ayyana a matsayin
karuwa na 0.003 a cikin ƙimar sha a 253nm ta hanyar enzymatic hydrolysis na BAEE a minti daya.
Amfanin Samfur
Don al'adun tantanin halitta
• Nama toshe narkewa, primary cell saye.
• Rage narkewar kwayoyin halitta.
• Al'adar salula ta hanyar microcarrier.
• Narkar da ƙwayoyin sel a hankali.
• Maganin rigakafi, da dai sauransu.
Domin gina jiki recombinant
• Recombinant samar da insulin.
• Jerin sunadaran sunadaran, taswirar peptide.
• Takamaiman matakai na proteolytic kamar binciken proteomics
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Bayyanar | Fari ko kashe farin foda |
| Solubility (10mg/ml) | Bayyana bayani mara launi |
| Tsafta | Β-trypsin ≥70%, α-trypsin ≤ 20% |
| Takamaiman Ayyuka (Foda) | ≥2500U/mg |
| E.Coli masaukin furotin ragowar | ≤0.01% |
| Endotoxins (LAL-Test) | ≤20 EU/mg |
Sufuri da ajiya
Sufuri:Ana aika shi a ƙasa da 2-8 ° C
Ajiya:Adana a -2-8°C, bayan narkar da 1mM hcl ko 50nM HAC, yakamata a adana a ƙarƙashin -25 ~ -15 °C
Nasihar sake gwada Rayuwa:shekara 2
