Hotunan da aka Fitar da Ƙwararriyar Nukiliya (ELISA).

Ultra Nuclease Assay Kit (ELISA)

Saukewa: 9025-65-4

EC.No.: 3.1.30.2

Kunshin: 96 Gwaji

Aika tambaya

Cikakken Bayani

Bayani

Wannan Ultra Nuclease kit ELISA yashi ne wanda ELISA za a yi a cikin sigar microplate.Samfurin mai yuwuwar ƙunshe da endonuclease ana shigar da shi a cikin rijiyoyin farantin microtiter waɗanda aka riga aka yi musu rufi tare da tsaftataccen maganin anti-endonuclease kama antibody.Bayan shiryawa da matakin wankewa wanda aka cire abubuwan da ba a ɗaure su ba, an ƙara wani ƙwayar enzyme-conjugated, anti-endonuclease detector antibody.Hanyoyin rigakafi suna haifar da samuwar hadadden sanwici na ingantaccen lokaci antibody-endonuclease-enzyme mai lakabin antibody.Bayan matakin wankewa na ƙarshe, an ƙara wani bayani na substrate a cikin rijiyoyin kuma ya amsa, yana haifar da ci gaban launi.Ana auna ma'aunin gani na hoto ta hanyar hoto kuma ya yi daidai da na
Analyte maida hankali ba a cikin rijiyoyin.Za'a iya ƙididdige ƙaddamarwar endonuclease a cikin samfuran da ba a san su ba bisa ga daidaitaccen ma'auni.

Tsarin Sinadarai

Ma'anar Naúrar

Adadin enzyme da aka yi amfani da shi don canza ƙimar sha na △A260 da 1.0 a cikin minti 30
a 37 ° C, pH 8.0, daidai da narkar da 37μg salmon maniyyi DNA ta yankan cikin oligonucleotides, an bayyana a matsayin mai aiki naúrar.

Amfani da Dosage

• Cire exogenous nucleic acid daga samfuran alurar riga kafi, rage haɗarin sauran guba na acid nucleic da inganta amincin samfur.
• Rage dankowar ruwan abinci da sinadarin nucleic acid ke haifarwa, rage lokacin aiki da kuma kara yawan furotin.
• Cire acid nucleic wanda ya nannade barbashi (virus, inclusion body, da dai sauransu), wanda ke taimakawa wajen saki da tsarkakewa.
• Maganin ƙwayoyin cuta na iya inganta ƙuduri da dawo da samfurin don chromatography na shafi, electrophoresis da bincike mai toshewa.
• A cikin maganin kwayoyin halitta, ana cire nucleic acid don samun ƙwayoyin cuta masu alaƙa da adeno.