Ultra Nuclease
Bayani
UltraNuclease wani endonuclaese ne da aka ƙera ta kwayoyin halitta wanda aka samo daga Serratia marcescens, wanda ke da ikon rage DNA ko RNA, ko dai sau biyu ko guda ɗaya, madaidaiciya ko madauwari a ƙarƙashin yanayi mai faɗi, gaba ɗaya ya lalata acid nucleic zuwa 5'-monophosphate oligonucleotides tare da 3-5 tsayin tushe.
Bayan gyare-gyaren aikin injiniya na kwayoyin halitta, samfurin ya kasance fermented, bayyana, da kuma tsarkakewa a cikin Escherichia coli (E. coli), wanda ya rage danko na sel supernatant da cell lysate a cikin binciken kimiyya, amma kuma inganta aikin tsarkakewa da kuma aikin bincike na gina jiki.Hakanan za'a iya amfani da shi a cikin maganin ƙwayoyin cuta, tsarkakewar ƙwayoyin cuta, samar da alluran rigakafi, furotin da masana'antar harhada magunguna ta polysaccharide azaman reagent ragowar nucleic acid.
Tsarin Sinadarai
Ma'anar Naúrar
Adadin enzyme da aka yi amfani da shi don canza ƙimar sha na △A260 ta 1.0 a cikin 30min a 37 ° C, pH 8.0, daidai da narkar da maniyyi na salmon 37μg DNA ta yankan cikin oligonucleotides, an bayyana shi azaman naúrar aiki.
Amfani da Dosage
• Cire exogenous nucleic acid daga samfuran alurar riga kafi, rage haɗarin sauran guba na acid nucleic da inganta amincin samfur.
• Rage dankowar ruwan abinci da sinadarin nucleic acid ke haifarwa, rage lokacin aiki da kuma kara yawan furotin.
• Cire acid nucleic wanda ya nannade barbashi (virus, inclusion body, da dai sauransu), wanda ke taimakawa wajen saki da tsarkakewa.
• Maganin ƙwayoyin cuta na iya inganta ƙuduri da dawo da samfurin don chromatography na shafi, electrophoresis da bincike mai toshewa.
• A cikin maganin kwayoyin halitta, ana cire nucleic acid don samun ƙwayoyin cuta masu alaƙa da adeno.
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Bayani | Bayyananne kuma mara launi |
| Ayyuka | ≥ 250 U/ul |
| Takamaiman ayyuka | ≥1.1*106U/mg |
| Tsaftace (SDS-PAGE) | ≥ 99.0% |
| Cutar cututtuka | Babu wanda aka gano |
| Kwayoyin halitta | 10 cfu/100,000U |
| Endotoxines (LAL-gwajin) | 0.25EU/1,000U |
Sufuri da ajiya
Sufuri:An aika a ƙarƙashin 0 ° C
Ajiya:Adana a -25 ~ -15 ° C
Nasihar sake gwada Rayuwa:shekara 2 (kauce wa daskarewa-narkewa)
