Alkaline Phosphatase (ALP)

Alkaline Phosphatase an samo shi daga recombinant E. coli iri wanda ke dauke da kwayar halittar TAB5.Enzyme yana haifar da dephosphorylation na 5' da 3' ƙarshen DNA da RNA phosphomonoesters.Har ila yau, yana lalata ribose, da kuma deoxyribonucleoside triphosphates (NTPs da dNTPs).TAB5 Alkaline Phosphatase yana aiki akan 5′ fidda kai, 5′ da tsautsayi da ƙarewa.Ana iya amfani da Phosphatase a aikace-aikacen ilimin halitta da yawa, irin su cloning ko alamar ƙarshen bincike don cire ƙarshen phosphorylated na DNA ko RNA.A cikin gwaje-gwajen cloning, dephosphorylation yana hana DNA plasmid layin da aka tsara daga haɗa kai.Hakanan yana iya lalata dNTPs marasa haɗin gwiwa a cikin halayen PCR don shirya samfuri don jerin DNA.Enzyme gabaɗaya kuma ba zai sake dawowa ba ta dumama a 70°C na tsawon mintuna 5, ta yadda za a cire phosphatase ɗin kafin a haɗa shi ko kuma ya ƙare ba dole ba.