ELEISA KIT don Trypsin
Bayani
Ana amfani da Recombinant Trypsin akai-akai a masana'antar biopharmaceutical-lokacin shirye-shiryen tantanin halitta ko don gyarawa da kunna samfuran.Trypsin yana haifar da haɗari na aminci don haka dole ne a cire shi kafin sakin samfurin ƙarshe.Wannan Kit ɗin Sanwici don auna ƙididdigewa ne na Residual Trypsin a cikin al'adun tantanin halitta mai girma da sauran hanyoyin masana'antar biopharmaceutical lokacin amfani da Trypsin.
Wannan kit ɗin shine Enzyme-Linked Immunosorbent Assay (ELISA).An riga an lulluɓe farantin da Porcine trypsin antibody.Trypsin da ke cikin samfurin ana ƙara kuma yana ɗaure ga ƙwayoyin rigakafi da aka lulluɓe akan rijiyoyin.Sannan ana ƙara maganin antibody na Porcine trypsin kuma yana ɗaure da trypsin a cikin samfurin.Bayan wankewa, ana ƙara HRP-Streptavidin kuma yana ɗaure ga antibody biotinylated trypsin.Bayan shiryawa unbound HRP-Streptavidin an wanke shi.Sa'an nan kuma TMB substrate bayani da aka kara da catalyzed ta HRP don samar da wani blue launi samfurin canza zuwa rawaya bayan ƙara acidic stop solution.Yawan rawaya ya yi daidai da adadin da aka yi niyya na trypsin
samfurin kama a faranti.Ana auna abin sha a 450 nm.
Tsarin Sinadarai
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Bayyanar | Cikakkun tattarawa kuma babu ɗigon ruwa |
| Ƙananan iyaka na ganowa | 0.003 ng/ml |
| Ƙananan iyaka na ƙididdigewa | 0.039 ng/ml |
| Daidaitawa | Intra Assay CV≤10% |
Sufuri da ajiya
Sufuri:yanayi
Ajiya:Za a iya adana shi a -25 ~ -15 ° C a rayuwar shiryayye, 2-8 ° C don sauran dacewa na gwaji.
Nasihar sake gwada Rayuwa:shekara 1
