Ribonuclease III (RNase III)
Bayani
Wannan samfurin shine ribonuclease III (RNase III) wanda E. coli ya sake bayyanawa.Wannan takamaiman exonuclease yana raba RNA mai madauri biyu (dsRNA) kuma yana haifar da gutsuttsuran 12-35bp dsRNA tare da 5'-PO4 da 3'-OH, 3' overhangs
Tsarin Sinadarai
Ma'anar Naúrar
Ma'anar sashin ayyuka: Raka'a ɗaya tana nufin adadin enzyme da ake buƙata don rage 1 μg na
dsRNA zuwa siRNA a cikin tsarin amsawa na 50 μL a 37°C na mintuna 20.
Ƙayyadaddun bayanai
| Kayan Gwaji | Ƙayyadaddun bayanai |
| Ayyukan Exonuclease | Yana fitar da 0.1% na jimlar aikin rediyo |
| Ayyukan DNA marasa takamaiman | Ba a iya ganowa |
| Gwajin Tsaftar Protein (SDS-PAGE) | ≥ 95% |
| Ayyukan RNase (Tsarin Narkewa) | 90% na substrate RNA ya kasance cikakke |
Sufuri da ajiya
Sufuri:Busasshen ƙanƙara
Ajiya:Adana a -25 ~ -15 ° C
Nasihar sake gwada Rayuwa:shekara 2
samfurori masu dangantaka
Ku rubuta sakonku anan ku aiko mana
